| Multidrug resistance gene expression correlates with progesterone production in dehydroepiandrosterone-induced polycystic and equine chorionic gonadotropin-stimulated ovaries of prepubertal rats. | |
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MedLine Citation:
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PMID: 9475386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polycystic ovaries (PCO) can be induced in prepubertal rats by daily injection of dehydroepiandrosterone (DHEA). There are high levels of progesterone, androgens, and estrogens in the cystic fluid of DHEA-treated rat ovaries. The purpose of this study was to investigate whether high levels of steroids in the PCO correlate with the expression of multidrug resistance gene product P-glycoprotein (Pgp). Using C219, a monoclonal antibody that recognizes the 170-kDa ATP-dependent transmembrane pump, we localized Pgp on the plasma membrane of granulosa cells in cystic follicles but not of oocytes or thecal/interstitial cells. In normal prepubertal rats, Pgp was localized in progesterone-producing granulosa cells of the preovulatory follicles and in cells of the corpora lutea after eCG/hCG stimulation, but not in growing follicles, oocytes, or thecal/interstitial cells. Northern analysis of these tissues indicated strong expression of Pgp mRNA in the preovulatory follicles, cystic follicles, and corpora lutea. From these findings it seems that progesterone produced by the granulosa cells may act in an autocrine manner to induce the expression of Pgp. It may be possible that progesterone interacts with the Pgp of these granulosa cells to modulate steroid efflux. |
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Authors:
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G Y Lee; J M Croop; E Anderson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 58 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-30 Completed Date: 1998-03-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 330-7 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Children's Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Chorionic Gonadotropin / pharmacology* Dehydroepiandrosterone / pharmacology* Female Gene Expression / physiology* Genes, MDR / physiology* Immunohistochemistry Ovary / drug effects, metabolism*, pathology P-Glycoprotein / biosynthesis Polycystic Ovary Syndrome / chemically induced, metabolism*, pathology Polymerase Chain Reaction Progesterone / biosynthesis* Radioimmunoassay Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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HD14574/HD/NICHD NIH HHS; HD28138/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/P-Glycoprotein; 53-43-0/Dehydroepiandrosterone; 57-83-0/Progesterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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