| Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. | |
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MedLine Citation:
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PMID: 7665473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutations in the secretory (sec) genes in Escherichia coli compromise protein translocation across the inner membrane and often confer conditional-lethal phenotypes. We have found that overproduction of the chaperonins GroES and GroEL from a multicopy plasmid suppresses a wide array of cold-sensitive sec mutations in E. coli. Suppression is accompanied by a stimulation of precursor protein translocation. This multicopy suppression does not bypass the Sec pathway because a deletion of secE is not suppressed under these conditions. Surprisingly, progressive deletion of the groE operon does not completely abolish the ability to suppress, indicating that the multicopy suppression of cold-sensitive sec mutations is not dependent on a functional groE operon. Indeed, overproduction of proteins unrelated to the process of protein export suppresses the secE501 cold-sensitive mutation, suggesting that protein overproduction, in and of itself, can confer mutations which compromise protein synthesis and the observation that low levels of protein synthesis inhibitors can suppress as well. In all cases, the mechanism of suppression is unrelated to the process of protein export. We suggest that the multicopy plasmids also suppress the sec mutations by compromising protein synthesis. |
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Authors:
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P N Danese; C K Murphy; T J Silhavy |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 177 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1995 Sep |
Date Detail:
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Created Date: 1995-10-11 Completed Date: 1995-10-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4969-73 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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biosynthesis*,
genetics,
metabolism Base Sequence Biological Transport Chaperonin 10 / biosynthesis, genetics Chaperonin 60 / biosynthesis, genetics Escherichia coli / genetics* Escherichia coli Proteins* Gene Dosage* Genetic Vectors Membrane Proteins* Membrane Transport Proteins* Molecular Sequence Data Mutagenesis / genetics* Plasmids / genetics Sequence Deletion Suppression, Genetic* beta-Galactosidase / biosynthesis, genetics |
| Grant Support | |
ID/Acronym/Agency:
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GM 14357-02/GM/NIGMS NIH HHS; GM07388/GM/NIGMS NIH HHS; GM41883/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Chaperonin 10; 0/Chaperonin 60; 0/Escherichia coli Proteins; 0/Membrane Proteins; 0/Membrane Transport Proteins; 0/SecD protein, E coli; 0/SecF protein, E coli; 0/secD protein, Bacteria; 0/secF protein, Bacteria; EC 3.2.1.23/beta-Galactosidase |
| Comments/Corrections | |
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