| Multicopy plasmids are clustered and localized in Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 11274369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We localized the multicopy plasmid RK2 in Escherichia coli and found that the number of fluorescent foci observed in each cell was substantially less than the copy number of the plasmid, suggesting that many copies of RK2 are grouped into a few multiplasmid clusters. In minimal glucose media, the majority of cells had one or two foci, with a single focus localized near midcell, and two foci near the 1/4 and 3/4 cell positions. The number of foci per cell increased with cell length and with growth rate, and decreased upon entering stationary phase, suggesting a coordination of RK2 replication or segregation with the bacterial cell cycle. Time-lapse microscopy demonstrated that partitioning of RK2 foci is achieved by the splitting of a single focus into two or three smaller foci, which are capable of separating with rapid kinetics. A derivative of the high-copy-number plasmid pUC19 containing the lacO array was also localized by tagging with GFP-LacI. Whereas many of the cells contained numerous, randomly diffusing foci, most cells exhibited one or two plasmid clusters located at midcell or the cell quarter positions. Our results suggest a model in which multicopy plasmids are not always randomly diffusing throughout the cell as previously thought, but can be replicated and partitioned in clusters targeted to specific locations. |
| | |
Authors:
|
J Pogliano; T Q Ho; Z Zhong; D R Helinski |
Related Documents
:
|
1937759 - Recombinant escherichia coli clones expressing chlamydia trachomatis gene products atta... 17277079 - Light-powering escherichia coli with proteorhodopsin. 16518839 - The influence of homogenisation conditions on biomass-adsorbent interactions during ion... 4106219 - Some effects of temperature on the growth of f pili. 334259 - The dynamic structure of the escherichia coli cell envelope as probed by 15n nuclear ma... 19285859 - Immobilization of bioluminescent escherichia coli cells using natural and artificial fi... 2543159 - Detection of hepatitis a virus rna and capsid antigen in individual cells. 20570859 - Proteomic cell surface phenotyping of differentiating acute myeloid leukemia cells. 3335499 - Isolation and characterization of chinese hamster ovary cell mutants deficient in acyl-... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2001-03-27 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 98 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2001 Apr |
Date Detail:
|
Created Date: 2001-04-11 Completed Date: 2001-05-10 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 4486-91 Citation Subset: IM |
Affiliation:
|
Division of Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92130-0322, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins
/
genetics Base Sequence DNA Primers Escherichia coli / genetics* Escherichia coli Proteins* Green Fluorescent Proteins Lac Repressors Luminescent Proteins / genetics Plasmids* Repressor Proteins / genetics Subcellular Fractions / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AI-07194/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/DNA Primers; 0/Escherichia coli Proteins; 0/Lac Repressors; 0/LacI protein, E coli; 0/Luminescent Proteins; 0/Repressor Proteins; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Myc represses the p21(WAF1/CIP1) promoter and interacts with Sp1/Sp3.
Next Document: Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoi...