| Multicolor spectral karyotyping identifies novel translocations in childhood acute lymphoblastic leukemia. | |
| | |
MedLine Citation:
|
PMID: 11237073 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We used a recently described molecular cytogenetic method, spectral karyotyping (SKY), to analyze metaphase chromosomes from 30 pediatric patients with acute lymphoblastic leukemia (ALL). This group included 20 patients whose leukemic blast cells lacked chromosomal abnormalities detected by conventional cytogenetics and 10 patients whose blast cells had multiple chromosomal abnormalities that could not be completely identified by G-banding analysis. In two of the 20 patients (10%) with apparently normal karyotypes, SKY identified three cryptic translocations: a t(7;8)(q34-35;q24.1) in one patient and a t(13;17)(q22;q21) and a der(19)t(17;19)(q22;p13) in another. Fluorescence in situ hybridization using subtelomeric probes proved the latter translocation to be a t(17;19). SKY analysis was also successful in defining the nature of the chromosomal abnormalities in four of the 10 patients with marker and derivative chromosomes. The identified abnormalities in the latter group included three novel translocations: a der(X)t(X;5)(p11.4;q31), a der(21)t(X;21)(p11.4;p11.2) and a t(X;9)(p11.4;p13). The presence of the t(X;9) was suggested by conventional cytogenetics. The application of fluorescence in situ hybridization using chromosome-specific painting probes and locus-specific probes complemented the SKY analysis by confirming the nature of the chromosome rearrangements defined by SKY and by identifying the amplification of the AML1/CBFA2 gene in one patient with a duplicated 21q. Our study demonstrates the utility of SKY in identifying novel translocations and in refining the identity of chromosomal abnormalities in leukemias. |
| | |
Authors:
|
S Mathew; P H Rao; J Dalton; J R Downing; S C Raimondi |
Related Documents
:
|
8453103 - The 8;21 chromosome translocation in acute myeloid leukemia is always detectable by mol... 11170283 - Isodicentric 7p, idic(7)(q11.2), in acute myeloid leukemia associated with older age an... 16681653 - Abortive second meiosis detected in cytochalasin-treated eggs in androgenetic diploid c... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Leukemia Volume: 15 ISSN: 0887-6924 ISO Abbreviation: Leukemia Publication Date: 2001 Mar |
Date Detail:
|
Created Date: 2001-03-09 Completed Date: 2001-03-29 Revised Date: 2013-03-04 |
Medline Journal Info:
|
Nlm Unique ID: 8704895 Medline TA: Leukemia Country: England |
Other Details:
|
Languages: eng Pagination: 468-72 Citation Subset: IM |
Affiliation:
|
Department of Pathology, St Jude Children's Research Hospital, Memphis, TN 38105-2794, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Child Child, Preschool Humans In Situ Hybridization, Fluorescence Infant Karyotyping / methods* Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics* Translocation, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
|
CA-20180/CA/NCI NIH HHS; CA-21765/CA/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pros and cons of splenectomy in patients with myelofibrosis undergoing stem cell transplantation.
Next Document: Treating onychomycosis.