| Multicolor flow cytometry analyses of cellular immune response in rhesus macaques. | |
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MedLine Citation:
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PMID: 20414189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rhesus macaque model is currently the best available model for HIV-AIDS with respect to understanding the pathogenesis as well as for the development of vaccines and therapeutics(1,2,3). Here, we describe a method for the detailed phenotypic and functional analyses of cellular immune responses, specifically intracellular cytokine production by CD4+ and CD8+ T cells as well as the individual memory subsets. We obtained precise quantitative and qualitative measures for the production of interferon gamma (INF-) and interleukin (IL) -2 in both CD4+ and CD8+ T cells from the rhesus macaque PBMC stimulated with PMA plus ionomycin (PMA+I). The cytokine profiles were different in the different subsets of memory cells. Furthermore, this protocol provided us the sensitivity to demonstrate even minor fractions of antigen specific CD4+ and CD8+ T cell subsets within the PBMC samples from rhesus macaques immunized with an HIV envelope peptide cocktail vaccine developed in our laboratory. The multicolor flow cytometry technique is a powerful tool to precisely identify different populations of T cells (4,5) with cytokine-producing capability(6) following non-specific or antigen-specific stimulation (5,7). |
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Authors:
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Hong He; Amy N Courtney; Eric Wieder; K Jagannadha Sastry |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Video-Audio Media Date: 2010-04-22 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2010 |
Date Detail:
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Created Date: 2010-04-23 Completed Date: 2010-04-30 Revised Date: 2012-04-23 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Immunology, The University of Texas M.D. Anderson Cancer Center, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Disease Models, Animal Flow Cytometry / methods* Immunity, Cellular Interferon-gamma / biosynthesis Interleukin-2 / biosynthesis Leukocytes, Mononuclear / immunology Macaca mulatta / blood, immunology* T-Lymphocyte Subsets / immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI46969/AI/NIAID NIH HHS; CA 16672/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-2; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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