Document Detail

Multicenter observational study on slow-cooling oocyte cryopreservation: clinical outcome.
MedLine Citation:
PMID:  20047739     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the efficacy of oocyte cryopreservation by a single slow-cooling protocol involving sucrose (0.2 mol/L) in the freezing solution. DESIGN: Observational comparison of the clinical outcome in fresh and frozen thawed cycles. SETTING: Public and private IVF centers. PATIENT(S): Infertile couples undergoing IVF treatment. INTERVENTION(S): Use of a maximum three oocytes in fresh cycles, as established by local law, and cryopreservation and later use of surplus oocytes. Likewise fresh cycles, maximum three thawed oocytes were used per cycle. All thawed oocytes were microinjected. MAIN OUTCOME MEASURE(S): Embryologic and clinical parameters of fresh and thawed cycles. RESULT(S): Two thousand forty-six patients underwent 2,209 oocyte retrievals involving oocyte cryopreservation. Overall, the survival rate of thawed oocytes was 55.8%. In 940 thaw cycles, the mean numbers of inseminated oocytes and fertilization rates were significantly decreased vs. fresh cycles outcomes (2.6 ± 0.7 vs. 2.9 ± 0.2 and 72.5% vs. 78.3%, respectively), as were the rates of implantation (10.1% vs. 15.4%), pregnancy rates per transfer (17.0% vs. 27.9%), and pregnancy rates per cycle (13.7% vs. 26.2%). Differences in clinical outcome were found among centers. A pregnancy rate per thawing cycle above 14% was achieved by most clinics. Fifty-seven retrievals involving oocyte cryopreservation achieved a pregnancy after fresh embryo replacement. Implantation and pregnancy rates per embryo transfer and per thawing cycles were 17.5%, 28.6%, and 24.6%, respectively. CONCLUSION(S): Under the conditions tested, the clinical outcome of oocyte slow-cooling cryopreservation is reduced compared with fresh cycles. Nevertheless, in cases of inapplicability of embryo cryopreservation, oocyte cryopreservation should be offered to patients with surplus oocytes.
Andrea Borini; Paolo Emanuele Levi Setti; Paola Anserini; Roberto De Luca; Lucia De Santis; Eleonora Porcu; Giovanni Battista La Sala; Annapia Ferraretti; Tiziana Bartolotti; Giovanni Coticchio; Giulia Scaravelli
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Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study     Date:  2010-01-04
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2010-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1662-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Tecnobios Procreazione, Centre for Reproductive Health, Bologna, Italy.
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MeSH Terms
Birth Rate*
Cell Survival / drug effects,  physiology
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Fertilization in Vitro / methods*
Middle Aged
Oocytes / cytology*,  drug effects,  physiology
Pregnancy Rate*
Retrospective Studies
Sucrose / pharmacology
Time Factors
Treatment Outcome
Reg. No./Substance:
0/Cryoprotective Agents; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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