| Multi-unit anti-BCR-ABL ribozyme therapy in chronic myelogenous leukemia. | |
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MedLine Citation:
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PMID: 8882949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this review, we summarize and update our data on the development of a multi-unit anti-BCR/ABL ribozyme. In vitro studies comparing several anti-BCR/ABL ribozymes demonstrated that a triple-unit ribozyme is the most efficient. Detailed kinetic analysis revealed this ribozyme to have a lower Kcat, most likely due to non homologous bases at restriction enzyme sites used in ribozyme construction. Delivery of this ribozyme to a BCR/ABL transformed cell line by a novel vehicle targeting the folate receptor resulted in a 3 log reduction in BCR/ABL mRNA when analyzed by RT-PCR. This delivery strategy reversed the IL-3 independence of this cell line. Retroviral vectors containing genes coding for the multi-unit ribozyme have been constructed and their use to effect BCR/ABL transformed cell biology is discussed. |
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Authors:
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L H Leopold; S K Shore; E P Reddy |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Leukemia & lymphoma Volume: 22 ISSN: 1042-8194 ISO Abbreviation: Leuk. Lymphoma Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1997-01-23 Completed Date: 1997-01-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 365-73 Citation Subset: IM |
Affiliation:
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Temple University Hospital, Philadelphia, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Fusion Proteins, bcr-abl / antagonists & inhibitors*, biosynthesis, genetics Gene Therapy* Humans Kinetics Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy* Molecular Sequence Data RNA, Catalytic / chemistry, metabolism, therapeutic use* RNA, Messenger / metabolism Substrate Specificity Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Fusion Proteins, bcr-abl; 0/RNA, Catalytic; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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