| Multi-parametric approach to identify coffee components that regulate mechanisms of gastric acid secretion. | |
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MedLine Citation:
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PMID: 22147653 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Scope: Chlorogenic acid (CA), caffeine (CAFF), pyrogallol (PYR), catechol (CAT), (β) N-alkanoyl-hydroxytryptamides (C5HT) and N-methylpyridinium (N-MP) were evaluated for their influence on mechanisms of gastric acid secretion as single compounds and in biomimetic mixtures. Methods and results: Compounds were tested in coffee representative concentrations. Human gastric cancer cells (HGT-1) were used to study the proton secretory activity by Ussing chamber experiments and FACS analysis. For activation of EGFr, Akt1, ERK1/2, ATF-2 and cAMP levels, we performed pathway screening assays. Time-dependent expression of related genes were determined by real-time PCR. Part of the data was used for neural network modeling to identify the most relevant compounds. N-MP increased the expression of the anti-secretory somatostatin receptor by 114%, whereas C5HT decreased its expression by 52%. N-MP down-regulated the pro-secretory CHRM3 receptor by 36% and the H(+) ,K(+) -ATPase by 36%. CAFF stimulated the secretory activity in the functional assays, whereas N-MP and CA decreased proton secretion. After applying a pathway analysis, we were able to discriminate between CAFF, CA, CAT, C5HT, PYR and histamine-activating EGFr signaling and N-MP-associated ERK1/2 signaling. Conclusion: By applying a multi-parametric approach, N-MP was shown to effectively down-regulate mechanisms of gastric acid secretion in human parietal gastric cells. |
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Authors:
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Malte Rubach; Roman Lang; Elisabeth Seebach; Mark M Somoza; Thomas Hofmann; Veronika Somoza |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-7 |
Journal Detail:
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Title: Molecular nutrition & food research Volume: - ISSN: 1613-4133 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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German Research Center for Food Chemistry, Freising, Germany; University of Wisconsin, Department of Food Science, Madison, WI, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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