| Multichanneled collagen conduits for peripheral nerve regeneration: design, fabrication, and characterization. | |
| | |
MedLine Citation:
|
PMID: 20528663 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
In the absence of donor tissues, conduits are needed for axons to regenerate across nerve defects, yet single-channel conduits may result in axonal dispersion, and multichannel synthetic polymer conduits have failed due to dimensional instability. The goal of this study was to create a robust collagen-based nerve conduit with multiple submillimeter-diameter channels to facilitate nerve guidance. Toward this goal, we have developed a novel multistep molding technique to create single-, four-, and seven-channel conduits from collagen and examined the effects of crosslinking with 0-60 mM (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide [EDC] in N-hydroxysuccinimide) on geometric, enzymatic, and thermal stability, mechanical properties, and cellular behavior. Multichannel collagen conduits crosslinked with 30 mM EDC and 10 mM N-hydroxysuccinimide demonstrated low degradation rate (∼10% at 2 days), high shrinkage temperature (>75°C), and constant channel morphology out to 30 days in saline. Neurite outgrowth remained unaffected from cultured dorsal root ganglia explants seeded on collagen scaffolds with up to 30 mM EDC crosslinking. Compared with single-channel conduits, multichannel collagen conduits showed superior structural compressive, tensile, and bending stiffness. Taken together, these results suggest that the crosslinked multichannel collagen conduits possess favorable material and mechanical properties for nerve regeneration applications. |
| | |
Authors:
|
Li Yao; Kristen L Billiar; Anthony J Windebank; Abhay Pandit |
Related Documents
:
|
16410023 - Zinc and copper: pharmacological probes and endogenous modulators of neuronal excitabil... 7065163 - A dual-channel signal averager for spontaneous signals. 10611943 - A sulfated proteoglycan aggregation factor mediates amyloid-beta peptide fibril formati... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-13 |
Journal Detail:
|
Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
|
Languages: eng Pagination: 1585-96 Citation Subset: IM |
Affiliation:
|
Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway, Ireland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: J.e. Grant and m.N. Potenzo (eds).
Next Document: Simple modular bioreactors for tissue engineering: a system for characterization of oxygen gradients...