| Multi-array silicon probes with integrated optical fibers: light-assisted perturbation and recording of local neural circuits in the behaving animal. | |
| | |
MedLine Citation:
|
PMID: 20529127 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recordings of large neuronal ensembles and neural stimulation of high spatial and temporal precision are important requisites for studying the real-time dynamics of neural networks. Multiple-shank silicon probes enable large-scale monitoring of individual neurons. Optical stimulation of genetically targeted neurons expressing light-sensitive channels or other fast (milliseconds) actuators offers the means for controlled perturbation of local circuits. Here we describe a method to equip the shanks of silicon probes with micron-scale light guides for allowing the simultaneous use of the two approaches. We then show illustrative examples of how these compact hybrid electrodes can be used in probing local circuits in behaving rats and mice. A key advantage of these devices is the enhanced spatial precision of stimulation that is achieved by delivering light close to the recording sites of the probe. When paired with the expression of light-sensitive actuators within genetically specified neuronal populations, these devices allow the relatively straightforward and interpretable manipulation of network activity. |
| | |
Authors:
|
Sébastien Royer; Boris V Zemelman; Mladen Barbic; Attila Losonczy; György Buzsáki; Jeffrey C Magee |
Related Documents
:
|
10576497 - Identification of hemifield single trial pvep on the basis of generalized dynamic neura... 7117447 - Central core control of developmental plasticity in the kitten visual cortex: ii. elect... 20329847 - The upper limit of temporal pitch for cochlear-implant listeners: stimulus duration, co... 15661107 - Electrode position and size in electrical impedance myography. 18561647 - Tumor trailing strategy for intensity-modulated radiation therapy of moving targets. 20870327 - Coexistence of myeloma cast nephropathy, light chain deposition disease, and nonamyloid... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-07 |
Journal Detail:
|
Title: The European journal of neuroscience Volume: 31 ISSN: 1460-9568 ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-07-08 Completed Date: 2011-01-25 Revised Date: 2011-03-03 |
Medline Journal Info:
|
Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France |
Other Details:
|
Languages: eng Pagination: 2279-91 Citation Subset: IM |
Affiliation:
|
Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Action Potentials
/
physiology Animals Behavior, Animal / physiology Electric Stimulation* / instrumentation, methods Electrophysiology* / instrumentation, methods Light* Mice Neurons / cytology, physiology* Neuropsychological Tests Optical Fibers* Photic Stimulation* / instrumentation, methods Rats Recombinant Fusion Proteins / genetics, metabolism Silicon / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
|
//Howard Hughes Medical Institute; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
|
0/Recombinant Fusion Proteins; 7440-21-3/Silicon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: TARPs gamma-2 and gamma-7 are essential for AMPA receptor expression in the cerebellum.
Next Document: Editorial.