Document Detail


Multi-armed cationic cyclodextrin:poly(ethylene glycol) polyrotaxanes as efficient gene silencing vectors.
MedLine Citation:
PMID:  23042106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A family of branched polyrotaxanes (bPRTx(+)), threaded with multiple cationic α-cyclodextrins (α-CDs) onto a multi-armed poly(ethylene glycol) (PEG) core, were synthesized and studied as gene silencing vectors. These bPRTx(+) formed stable, positively charged complexes with diameters of 150-250 nm at N/P ratios as low as 2.5. The bPRTx(+) materials were shown to have gene-silencing efficiencies comparable to those of Lipofectamine 2000 (L2k) and bPEI, while displaying similar toxicity profiles. The unique structure of these polyrotaxanes allows them to effectively condense and complex siRNA into nanoparticles at much lower N/P ratios than L2k or bPEI. These findings suggest that bPRTx(+) may be useful materials for gene therapy applications.
Authors:
Aditya Kulkarni; Kyle DeFrees; Ryan A Schuldt; Alexander Vlahu; Ross VerHeul; Seok-Hee Hyun; Wei Deng; David H Thompson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-06-24     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  115-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cations
Cyclodextrins / chemistry*
Gene Silencing*
Genetic Vectors / genetics
Mice
NIH 3T3 Cells
Nanocapsules / administration & dosage,  chemistry*
Poloxamer / chemistry*
Polyethylene Glycols / chemistry*
RNA, Small Interfering / administration & dosage*,  genetics*
Rotaxanes / chemistry*
Transfection / methods*
Grant Support
ID/Acronym/Agency:
CA23168/CA/NCI NIH HHS; GM087016/GM/NIGMS NIH HHS; R01 GM087016/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cations; 0/Cyclodextrins; 0/Nanocapsules; 0/Polyethylene Glycols; 0/RNA, Small Interfering; 0/Rotaxanes; 0/polyrotaxane; 106392-12-5/Poloxamer
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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