Document Detail

Multi-analyst, multi-matrix performance of the QuEChERS approach for pesticide residues in foods and feeds using HPLC/MS/MS analysis with different calibration techniques.
MedLine Citation:
PMID:  20480880     Owner:  NLM     Status:  MEDLINE    
Three different calibration approaches were applied in HPLC/MS/MS using electrospray ionization for the determination of 14 diverse pesticide residues at different levels in a variety of food matrixes. This study was conducted as part of a 4 day training course for 17 chemists to learn the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) approach to pesticide residue analysis in foods. The analysts were divided into four different teams for the analysis of 12 different matrixes (strawberries, plums, carrots, green peppers, milk, molasses, alfalfa oats, corn silage, dry pet food, soybean, almonds, and foliage). The acetate-buffered QuEChERS protocol gave excellent results in the spiked samples for all matrixes tested. The HPLC/MS/MS calibration techniques consisted of external standardization in solvent solutions, matrix-matching, and the echo-peak technique. Peak areas were normalized to an internal standard in all three approaches. Matrix effects were observed with the corn silage, carrot, and foliage extracts, but they were minimal or nonexistent in the other matrixes. Matrix-matching best compensated for matrix effects, but has logistical difficulties in real-world application and required extra sample preparation compared to the other approaches. The echo-peak technique reduced matrix effects but did not eliminate them.
Steven J Lehotay; Katerina Mastovska; Alan R Lightfield; Robert A Gates
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-67     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Food Analysis
Food Contamination
Pesticide Residues / analysis*
Reference Standards
Reproducibility of Results
Solvents / chemistry
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry / methods*
Time Factors
Reg. No./Substance:
0/Ions; 0/Pesticide Residues; 0/Solvents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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