Document Detail

Multi-Mycotoxin Analysis of Finished Grain and Nut Products Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
MedLine Citation:
PMID:  23614683     Owner:  NLM     Status:  Publisher    
Mycotoxins in foods have long been recognized as a potential health hazard due to their toxic and carcinogenic properties. A simple and rapid method was developed to detect mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes and ergot alkaloids) in corn, rice, wheat, almond, peanut, and pistachio. Test portions of homogenized grains or nuts were extracted with acetonitrile/water (85/15, v/v), followed by high-speed homogenization and centrifugation. The resulting supernatant was diluted with water and analyzed for twenty-six mycotoxins and five stable isotope labeled mycotoxins using high-performance liquid chromatography-positive electrospray ionization-tandem mass spectrometry (LC-MS/MS) in scheduled multiple reaction monitoring (sMRM) mode. Mean recoveries (± standard deviations) were 84 ± 6, 89 ± 6, 97 ± 9, 87 ± 12, 104 ± 16, and 92 ± 18% and the geometric mean limits of quantitation (LOQs) were 1.6, 1.4, 1.5, 1.4, 1.6, and 1.5 µg/kg for corn, rice, wheat, almond, peanut and pistachio, respectively. The matrix effects on each mycotoxin, as measured by the slope ratios of matrix-matched and solvent-only calibration curves, varied in each commodity ranging from -56% (suppression, i.e., ergotamine in peanut) to +57% (enhancement, i.e., beauvericin in almond) and were more pronounced in nuts than in grains. The measured mycotoxin concentrations in eleven corn and wheat reference materials were not statistically different from the certified concentrations with the exception of ochratoxin A in wheat. Mycotoxin concentrations in commercial and finished grain and nut samples were measured in 70 commercial samples. In these samples, 19 mycotoxins were identified at concentrations ranging from 0.3 ± 0.1 (aflatoxin B1 in peanuts) to 1143 ± 87 (fumonisin B1 in corn flour) µg/kg. Beauvericin, ergot alkaloids, fumonisins, ochratoxin A, trichothecenes, strerigmatocystin, and zearalenone were measured in corn, rice and wheat products, while aflatoxins, beauvericin, neosolaniol, and ochratoxins were measured in almond, peanut and pistachio products. This rapid and efficient method was shown to be rugged and effective for the multiresidue analysis of mycotoxins in grain and nut products.
Chia-Ding Liao; Jon W Wong; Kai Zhang; Douglas G Hayward; Nathaniel S Lee; Mary Wat Trucksess
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-24
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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