Document Detail


Mucus glycoprotein secretion by duodenal mucosa in response to luminal arachidonic acid.
MedLine Citation:
PMID:  3096377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of luminal application of arachidonic acid on the alkaline secretion, prostaglandin generation, and mucus glycoprotein output and composition was studied in proximal and distal duodenum of conscious dogs. Surgically prepared duodenal loops were instilled in vivo for up to 2 h with saline (control) followed by various concentrations (12.5-100 micrograms/ml) of arachidonic acid. The experiments were conducted with and without intravenous pretreatment with indomethacin. The recovered instillates were assayed for the content of prostaglandin and HCO3-, and used for the isolation of mucus glycoprotein. Exposure of duodenal mucosa to arachidonic acid led to concentration-dependent increase in the output of HCO3- and prostaglandin generation. In both cases this response was greater in the proximal duodenum. Pretreatment with indomethacin caused reduction in the basal HCO3- and prostaglandin output, and prevented the increments evoked by arachidonic acid. The proximal and distal duodenum displayed similar basal output and composition of mucus glycoprotein. Comparable increases in these glycoproteins were also obtained with arachidonic acid, the effect of which was abolished by indomethacin. Compared to basal conditions, mucus glycoproteins elaborated in response to arachidonic acid exhibited higher contents of associated lipids and covalently bound fatty acids, and contained less protein. The associated lipids of mucus glycoproteins elaborated in the presence of arachidonic acid showed enrichment in phospholipids and decrease in neutral lipids. The carbohydrate components in these glycoproteins also exhibited higher proportions of sialic acid and sulfate. The changes brought about by arachidonic acid were prevented by indomethacin pretreatment, and in both cases the glycoprotein composition returned to that obtained under basal conditions. The enrichment of mucus glycoprotein in lipids, sialic acid and sulfate in response to endogenous prostaglandin may be of significance to the function of this glycoprotein in the hostile environment of the duodenum.
Authors:
M Kosmala; S R Carter; S J Konturek; A Slomiany; B L Slomiany
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  884     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-22     Completed Date:  1987-01-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  419-28     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid
Arachidonic Acids / pharmacology*
Dogs
Duodenum / drug effects,  secretion*
Glycoproteins / isolation & purification,  secretion*
Hydrogen-Ion Concentration
Indomethacin / pharmacology
Intestinal Mucosa / drug effects,  secretion*
Organ Specificity
Perfusion
Grant Support
ID/Acronym/Agency:
AM21684-09/AM/NIADDK NIH HHS; HL32553-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Glycoproteins; 0/gastric mucus glycoproteins; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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