Document Detail

Mucoadhesive and penetration enhancement properties of three grades of hyaluronic acid using porcine buccal and vaginal tissue, Caco-2 cell lines, and rat jejunum.
MedLine Citation:
PMID:  15324476     Owner:  NLM     Status:  MEDLINE    
The influence of the molecular weight on mucoadhesive and penetration enhancement properties of three grades of hyaluronic acid (1878, 693 and 202 kDa) has been evaluated. The mucoadhesive properties were investigated using buccal and vaginal porcine mucosa by means of a tensile stress method and using rat jejunum by means of an inclined plane method. The mucoadhesive performances observed using animal tissues were compared with the mucoadhesive properties observed using submaxillary or gastric mucin dispersions. The penetration enhancement properties were investigated using porcine buccal epithelium membrane or vaginal tissue and a cell monolayer (Caco-2 cell line). Chitosan hydrochloride, already described as a penetration enhancer towards buccal and vaginal mucosae and Caco-2 cell monolayers, was used as reference. Aciclovir (acyclovir), a poorly soluble and absorbable drug, commonly used in the treatment of Herpes simplex virus (type I and II), was used as the model drug. Unlike chitosan hydrochloride, which does not show any mucoadhesive potential at pH close to neutrality (buccal and intestinal), all hyaluronic acid grades show mucoadhesive properties in all the environments considered (buccal, vaginal and intestinal). In all cases, a decrease in molecular weight of hyaluronic acid produced an increase in the mucoadhesive performance. The hyaluronic acid with the lowest molecular weight (202 kD) exhibited the best penetration enhancement properties, that, depending on the substrate under consideration, was either comparable with or even better than chitosan hydrochloride. Therefore, this grade would be the most promising for buccal, vaginal and intestinal delivery of aciclovir.
Giuseppina Sandri; Silvia Rossi; Franca Ferrari; Maria Cristina Bonferoni; Naima Zerrouk; Carla Caramella
Related Documents :
20148096 - The role of different hyaluronic acids in the articular cartilage of rabbit.
3519016 - Aromatic l-amino acid decarboxylase: histochemical localization in rat kidney and lack ...
6952186 - Ultrastructure of a hyaluronic acid matrix.
10523596 - Recovery of mycobacteria from patients with cystic fibrosis.
3451956 - Effect of allopurinol on the intestinal absorption of 6-mercaptopurine in rats.
237966 - Analysis of lipid composition of isolated human sebaceous gland homogenates after incub...
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  56     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-24     Completed Date:  2004-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1083-90     Citation Subset:  IM    
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adhesives / chemistry,  pharmacokinetics*
Caco-2 Cells
Hyaluronic Acid / chemistry,  pharmacokinetics*
Jejunum / metabolism*
Mouth Mucosa / metabolism*
Permeability / drug effects
Vagina / metabolism*
Reg. No./Substance:
0/Adhesives; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A review of selected anti-tumour therapeutic agents and reasons for multidrug resistance occurrence.
Next Document:  Efficacy of chitosan microspheres for controlled intra-articular delivery of celecoxib in inflamed j...