Document Detail


Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation.
MedLine Citation:
PMID:  15671211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mucous layer that covers the intestinal absorptive surface acts as a barrier against bacterial translocation. The chicken gut contains a diverse bacterial population which interacts with the mucous layer. In this report, we studied the effect of changing the intestinal microbial populations on mucin dynamics by feeding 1-d-old chicks a control diet or that diet containing either antibiotic growth promoter (AGP) or a probiotic product for 14 d. Dietary AGP increased the proportions of Bifidobacterium species in the duodenum compared with the other groups. In AGP-fed chicks, the villous surface area was increased in the jejunum, goblet cell density was greater in the jejunum and ileum, and mucin glycoprotein levels in the duodenum were lower than in the other groups (P < 0.05). Feeding AGP increased the expression of mucin mRNA in the jejunum and ileum compared with controls. The dietary probiotic increased the proportion of Lactobacillus species in the ileum compared with the controls (P < 0.05) and significantly enlarged the goblet cell "cup" area throughout the small intestine compared with the other groups. Expression of mucin mRNA and the levels of mucin glycoprotein were greater in the jejunum of the probiotic-fed chicks compared with controls (P < 0.05). Neither the probiotic nor AGP treatments affected the thickness of the mucous adherent layer. These results indicate that both probiotic and AGP altered processes of mucin biosynthesis and/or degradation mediated via changes in the intestinal bacterial populations. These modifications in mucin dynamics influence gut function and health and may change nutrient uptake.
Authors:
A Smirnov; R Perez; E Amit-Romach; D Sklan; Z Uni
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  135     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-26     Completed Date:  2005-04-14     Revised Date:  2006-04-13    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Affiliation:
The Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Anti-Bacterial Agents / administration & dosage,  pharmacology*
Bifidobacterium / drug effects,  growth & development
Chickens
DNA Primers
DNA, Bacterial / genetics,  isolation & purification
Diet*
Dietary Supplements
Enterococcus faecium / drug effects,  growth & development
Female
Genome, Bacterial
Growth / drug effects
Intestinal Mucosa / drug effects,  microbiology,  physiology
Intestine, Small / drug effects,  microbiology*,  physiology*
Lactobacillus / drug effects,  growth & development
Male
Mucins / metabolism*
Polymerase Chain Reaction
Probiotics
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA Primers; 0/DNA, Bacterial; 0/Mucins

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