Document Detail


Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus.
MedLine Citation:
PMID:  21049484     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%) B(max) values for the mesial TLE group, whereas the K(d) values were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO-stimulated [(35) S]GTPγS binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations. © 2010 Wiley Periodicals, Inc.
Authors:
Manola Cuellar-Herrera; Ana Luisa Velasco; Francisco Velasco; Laura Chavez; Sandra Orozco-Suarez; Guliz Armagan; Ezgi Turunc; Engin Bojnik; Ayfer Yalcin; Sandor Benyhe; Anna Borsodi; Mario Alonso-Vanegas; Luisa Rocha
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Publication Detail:
Type:  Journal Article     Date:  2010-11-03
Journal Detail:
Title:  Hippocampus     Volume:  22     ISSN:  1098-1063     ISO Abbreviation:  Hippocampus     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9108167     Medline TA:  Hippocampus     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley Periodicals, Inc.
Affiliation:
Epilepsy Clinic of Hospital General de Mexico, Mexico City, Mexico.
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