| MsrA protects cardiac myocytes against hypoxia/reoxygenation induced cell death. | |
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MedLine Citation:
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PMID: 18083115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reactive oxygen species (ROS) are critical in tissue responses to ischemia-reperfusion. The enzyme methionine sulfoxide reductase-A (MsrA) is capable of protecting cells against oxidative damage by reversing damage to proteins caused by methionine oxidation or by decreasing ROS through a scavenger mechanism. The current study employed adenovirus mediated over-expression of MsrA in primary neonatal rat cardiac myocytes to determine the effect of this enzyme in protecting against hypoxia/reoxygenation in this tissue. Cells were transduced with MsrA encoding adenovirus and subjected to hypoxia/reoxygenation. Apoptotic cell death was decreased by greater than 45% in cells over-expressing MsrA relative to cells transduced with a control virus. Likewise total cell death as determined by levels of LDH release was dramatically decreased by MsrA over-expression. These observations indicate that MsrA is protective against hypoxia/reoxygenation stress in cardiac myocytes and point to MsrA as an important therapeutic target for ischemic heart disease. |
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Authors:
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H M Prentice; I A Moench; Z T Rickaway; C J Dougherty; K A Webster; H Weissbach |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-18 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 366 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-08 Completed Date: 2008-02-05 Revised Date: 2012-05-28 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 775-8 Citation Subset: IM |
Affiliation:
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Florida Atlantic University, College of Biomedical Science, 777 Glades Road, Boca Raton, FL 33431, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apoptosis Cardiotonic Agents / metabolism* Cell Hypoxia Cells, Cultured Myocytes, Cardiac / metabolism*, pathology* Oxidoreductases / metabolism* Rats Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cardiotonic Agents; 0/Reactive Oxygen Species; EC 1.-/Oxidoreductases; EC 1.8.4.11/MsrA protein, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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