| MsGC-II, a receptor guanylyl cyclase isolated from the CNS of Manduca sexta that is inhibited by calcium. | |
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MedLine Citation:
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PMID: 12558998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the cloning of a receptor guanylyl cyclase, MsGC-II, from the CNS of the insect Manduca sexta. Sequence comparisons with other receptor guanylyl cyclases show that MsGC-II is most similar to a predicted guanylyl cyclase in the Drosophila genome and to vertebrate retinal guanylyl cyclases. When expressed in COS-7 cells, MsGC-II exhibited a low level of basal activity that was nearly abolished in the presence of 10 micro m calcium. Incubation with either a mammalian guanylyl cyclase-activating protein or Drosophila frequenin resulted in only mild stimulation of activity, whereas incubation of COS-7 cells expressing MsGC-II with a variety of Manduca tissue extracts failed to stimulate enzyme activity above basal levels. Analysis of the tissue distribution of MsGC-II revealed that it is nervous system specific. In the adult, MsGC-II is present in neurons in the optic lobes, antennal lobes and cellular cortex, but it is most highly expressed in subsets of intrinsic mushroom body neurons. Thus, MsGC-II appears to be a neural-specific receptor guanylyl cyclase whose activity may be regulated either directly or indirectly by calcium. |
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Authors:
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David B Morton; Alan Nighorn |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 84 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-31 Completed Date: 2003-02-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 363-72 Citation Subset: IM |
Affiliation:
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Department of Biological Structure and Function, Oregon Health and Science University, Portland 97201, USA. mortonda@ohsu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Calcium / metabolism, pharmacology* Central Nervous System / chemistry, enzymology* Cloning, Molecular Drosophila Guanylate Cyclase / antagonists & inhibitors*, genetics, isolation & purification Manduca / enzymology* Molecular Sequence Data Mushroom Bodies / cytology, metabolism Neurons / metabolism Organ Specificity Receptors, Cell Surface / antagonists & inhibitors, genetics, isolation & purification Sequence Homology, Amino Acid Transfection |
| Grant Support | |
ID/Acronym/Agency:
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DC04292/DC/NIDCD NIH HHS; NS29740/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Cell Surface; 7440-70-2/Calcium; EC 4.6.1.2/Guanylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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