| The Moving Boundary Node Method: A level set-based, finite volume algorithm with applications to cell motility. | |
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MedLine Citation:
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PMID: 20689723 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Eukaryotic cell crawling is a highly complex biophysical and biochemical process, where deformation and motion of a cell are driven by internal, biochemical regulation of a poroelastic cytoskeleton. One challenge to building quantitative models that describe crawling cells is solving the reaction-diffusion-advection dynamics for the biochemical and cytoskeletal components of the cell inside its moving and deforming geometry. Here we develop an algorithm that uses the level set method to move the cell boundary and uses information stored in the distance map to construct a finite volume representation of the cell. Our method preserves Cartesian connectivity of nodes in the finite volume representation while resolving the distorted cell geometry. Derivatives approximated using a Taylor series expansion at finite volume interfaces lead to second order accuracy even on highly distorted quadrilateral elements. A modified, Laplacian-based interpolation scheme is developed that conserves mass while interpolating values onto nodes that join the cell interior as the boundary moves. An implicit time-stepping algorithm is used to maintain stability. We use the algoirthm to simulate two simple models for cellular crawling. The first model uses depolymerization of the cytoskeleton to drive cell motility and suggests that the shape of a steady crawling cell is strongly dependent on the adhesion between the cell and the substrate. In the second model, we use a model for chemical signalling during chemotaxis to determine the shape of a crawling cell in a constant gradient and to show cellular response upon gradient reversal. |
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Authors:
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Charles W Wolgemuth; Mark Zajac |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of computational physics Volume: 229 ISSN: 0021-9991 ISO Abbreviation: - Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-8-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9883524 Medline TA: J Comput Phys Country: - |
Other Details:
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Languages: ENG Pagination: 7287-7308 Citation Subset: - |
Affiliation:
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Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030-3505. |
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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U54 RR022232-01//NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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