| Movements and interactions of leukocytes in microgravity. | |
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MedLine Citation:
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PMID: 8987569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mitogenic activation of human lymphocytes resuspended in vitro is dramatically reduced in microgravity. As cell-cell contacts are one of the elements essential for activation, the behaviour of human leukocytes (mainly lymphocytes and monocytes as accessory cells) in the presence of the mitogen concanavalin A was studied in the centrifuge microscope NIZEMI at 0 x g. Aggregates (formed by intercellular bindings of membrane glycoproteins via the tetravalent alpha-glucoside ligand concanavalin A) were found at 0 x g as well as at 1 x g already 12 h after the addition of the mitogen. In general, the aggregates observed at 0 x g after an incubation time of 46 and 78 h were smaller than the corresponding aggregates in the ground control. The findings are of primary importance since they confirm the indirect evidence we had from earlier Spacelab experiments and demonstrate that cell-cell contacts are occurring also in microgravity. In addition, single cells in 0 x g show a significant higher locomotion velocity than the cells at 1 x g. The fact that the locomotion capability is not decreased during the 78-h incubation with concanavalin A provides further evidence that the cells are not proceeding through the cell cycle. |
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Authors:
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M Cogoli-Greuter; M A Meloni; L Sciola; A Spano; P Pippia; G Monaco; A Cogoli |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biotechnology Volume: 47 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1997-02-19 Completed Date: 1997-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 279-87 Citation Subset: B; S |
Affiliation:
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Space Biology Group, Swiss Federal Institute of Technology, Technopark, Zürich, Switzerland. |
Space Flight Mission:
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Flight Experiment; STS-65 Shuttle Project; manned; short duration |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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instrumentation Cell Aggregation Cell Communication Cell Movement Concanavalin A / pharmacology Humans Leukocytes / cytology, immunology, physiology* Lymphocyte Activation Microscopy, Electron, Scanning Space Flight* / instrumentation Weightlessness / adverse effects* |
| Chemical | |
Reg. No./Substance:
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11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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