Document Detail


Movement of tomato yellow leaf curl geminivirus (TYLCV): involvement of the protein encoded by ORF C4.
MedLine Citation:
PMID:  8091687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the ability of a TYLCV DNA mutant containing a disrupted ORF C4 to infect Nicotiana benthamiana and tomato plants. The mutant retained the capability of autonomous replication in protoplast-derived cells of tomato and was able to infect N. benthamiana plants systemically although DNA levels were reduced and symptom development was attenuated. However, when tomato plants were inoculated, the virus was unable to move systemically unless a second site mutation or a reversion in planta restored the integrity of ORF C4. The infected plants remained asymptomatic or showed very mild symptoms. The results strongly suggest that the ORF C4 encodes a protein involved in virus movement, a novel finding for whitefly-transmitted geminiviruses. The involvement of a C4 protein in symptom determination is discussed.
Authors:
I Jupin; F De Kouchkovsky; F Jouanneau; B Gronenborn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virology     Volume:  204     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-20     Completed Date:  1994-10-20     Revised Date:  2001-11-28    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  82-90     Citation Subset:  IM    
Affiliation:
Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
DNA, Viral / analysis
Geminiviridae / genetics*,  physiology
Genes, Viral / genetics*
Molecular Sequence Data
Open Reading Frames / genetics*
Plant Diseases / microbiology*
Plants, Toxic
Point Mutation*
Protoplasts / microbiology
Tobacco / microbiology
Vegetables / microbiology
Viral Proteins / genetics*,  physiology
Virus Replication
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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