Document Detail


Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population.
MedLine Citation:
PMID:  22170865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: During gastrulation, an embryo acquires the three primordial germ layers that will give rise to all of the tissues in the body. In amniote embryos, this process occurs via an epithelial to mesenchymal transition (EMT) of epiblast cells at the primitive streak. Although the primitive streak is vital to development, many aspects of how it forms and functions remain poorly understood.
RESULTS: Using live, 4 dimensional imaging and immunohistochemistry, we have shown that the posterior epiblast of the pre-streak murine embryo does not display convergence and extension behavior or large scale migration or rearrangement of a cell population. Instead, the primitive streak develops in situ and elongates by progressive initiation EMT in the posterior epiblast. Loss of basal lamina (BL) is the first step of this EMT, and is strictly correlated with ingression of nascent mesoderm. Once the BL is lost in a given region, cells leave the epiblast by apical constriction in order to enter the primitive streak.
CONCLUSIONS: This is the first description of dynamic cell behavior during primitive streak formation in the mouse embryo, and reveals mechanisms that are quite distinct from those observed in other amniote model systems. Unlike chick and rabbit, the murine primitive streak arises in situ by progressive initiation of EMT beginning in the posterior epiblast, without large-scale movement or convergence and extension of epiblast cells.
Authors:
Margot Williams; Carol Burdsal; Ammasi Periasamy; Mark Lewandoski; Ann Sutherland
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-12-14
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  241     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-25     Completed Date:  2012-07-20     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  270-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Epithelial-Mesenchymal Transition*
Germ Layers / cytology,  physiology
Mice
Mice, Inbred Strains
Primitive Streak / cytology*,  physiology*
Signal Transduction
Grant Support
ID/Acronym/Agency:
R01 HD034807/HD/NICHD NIH HHS; R01 HD034807-07/HD/NICHD NIH HHS; T32 GM008136/GM/NIGMS NIH HHS; T32 GM008136-21/GM/NIGMS NIH HHS
Comments/Corrections

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