Document Detail

Mouse oocytes derived from fetal germ cells are competent to support the development of embryos by in vitro fertilization.
MedLine Citation:
PMID:  16868930     Owner:  NLM     Status:  MEDLINE    
Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature oocytes from the fetal mouse germ cells at 16.5 days post-coitum (dpc). For the initial of follicular formation, fetal mouse 16.5 dpc ovaries were transplanted to the recipient under the kidney capsule, and the ovaries were recovered after 14 days. Subsequently, the growing preantral follicles in the ovarian grafts were isolated and cultured in vitro for 12 days. Practically, the mature oocytes ovulated from the antral follicles were able to be fertilized in vitro and support the embryonic development. The results demonstrate that the fetal mouse 16.5 dpc germ cells are able to form primordial follicles with the ovarian pregranulosa cells during the period of transplantation in the ectopic site, and the oocytes within the growing follicles are able to mature in vitro, then are able to support the embryonic development.
Wei Shen; Lan Li; Donghui Zhang; Qingjie Pan; Mingxiao Ding; Hongkui Deng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  73     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2006-10-05     Revised Date:  2008-10-28    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1312-7     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
College of Life Sciences, Peking University, Beijing, China.
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MeSH Terms
Cell Differentiation
Embryonic Development*
Fertilization in Vitro*
Fetus / cytology
Germ Cells / cytology,  physiology
Mice, Inbred Strains
Oocytes / cytology,  physiology*
Ovarian Follicle / growth & development,  transplantation
Ovary / cytology,  transplantation
Erratum In:
Mol Reprod Dev. 2008 Nov;75(11):1688-9

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