| Mouse model of liver ischemia and reperfusion injury: method for studying reactive oxygen and nitrogen metabolites in vivo. | |
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MedLine Citation:
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PMID: 18955130 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mouse model of liver ischemia and reperfusion injury has proven to be valuable for our understanding of the role that reactive oxygen and nitrogen metabolites play in postischemic tissue injury. This methods paper provides a detailed protocol for inducing partial liver ischemia followed by reperfusion. Liver ischemia is induced in anesthetized mice by cross-clamping the hepatic artery and portal vein for varying lengths of time, resulting in deprivation of blood flow to approximately 70% of the liver. Restoration of blood flow to the ischemic lobes enhances superoxide production concomitant with a rapid and marked decrease in the bioavailability of nitric oxide, resulting in alterations in the redox state of the liver in favor of a more oxidative environment. This hepatocellular oxidative stress induces the activation of oxidant-sensitive transcription factors followed by the upregulation of proinflammatory cytokines and mediators that ultimately lead to liver injury. This model can be induced in any strain or sex of mouse and requires 1-2 months of practice to become proficient in the surgery and animal manipulation. The roles of various reactive metabolites of oxygen and nitrogen may be evaluated using genetically engineered mice as well as selective molecular, cellular, and/or pharmacological agents. |
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Authors:
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Yuta Abe; Ian N Hines; Gazi Zibari; Kevin Pavlick; Laura Gray; Yuko Kitagawa; Matthew B Grisham |
Publication Detail:
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Type: Journal Article Date: 2008-10-10 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 46 ISSN: 1873-4596 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-19 Completed Date: 2009-11-02 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, 71130, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Alanine Transaminase / blood, genetics Animals Animals, Inbred Strains Biochemistry / methods Disease Models, Animal* Enzyme Activation Liver / blood supply, enzymology*, pathology, surgery Mice Mice, Knockout Nitric Oxide / genetics, metabolism Nitric Oxide Synthase Type III / metabolism Protein Engineering Reperfusion / instrumentation, methods Reperfusion Injury / blood, enzymology*, pathology Species Specificity Superoxide Dismutase Superoxides / metabolism Time Factors Tumor Necrosis Factor-alpha / blood, genetics |
| Grant Support | |
ID/Acronym/Agency:
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P01 DK043785-070006/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.15.1.1/Sod3 protein, mouse; EC 1.15.1.1/Superoxide Dismutase; EC 1.15.1.1/superoxide dismutase 2; EC 2.6.1.2/Alanine Transaminase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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