| Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse. | |
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MedLine Citation:
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PMID: 15236182 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: The relationship between intestinal pathology and immune expulsion of gastrointestinal nematodes remains controversial. Immune expulsion of gastrointestinal helminth parasites is usually associated with Th2 responses, but the effector mechanisms directly responsible for parasite loss have not been elucidated. Mast cell hyperplasia is a hallmark of infection with gastrointestinal nematodes, in particular Trichinella spiralis. Although the precise mechanism by which mast cells induce expulsion of these parasites has not been elucidated, it has been proposed that mast cell mediators, including cytokines and granule chymases, act to create an environment inhospitable to the parasite, part of this being the induction of intestinal inflammation. Therefore, the aims of this study were to dissect the role of mast cells and mast cell proteases in the induction of parasite-induced enteropathy. METHODS: Mast cell-deficient W/Wv and mouse mast cell protease-1 (mMCP-1)-deficient mice were infected with T. spiralis, and parasite expulsion, enteropathy, and Th2 responses were determined. RESULTS: Expulsion of the parasite was delayed in both strains of mice compared with wild-type controls; additionally, in both cases, the enteropathy was significantly ameliorated. Although Th2 responses were significantly reduced in mast cell-deficient W/Wv mice, those from mMCP-1-deficient mice were similar to wild-type mice. Additionally, levels of TNF-alpha and nitric oxide were significantly reduced in both W/Wv and mMCP-1 deficient mice. CONCLUSIONS: These results imply that mast cells may contribute to the induction of protective Th2 responses and, importantly, that the intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1. |
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Authors:
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Catherine E Lawrence; Yvonne Y W Paterson; Steven H Wright; Pamela A Knight; Hugh R P Miller |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gastroenterology Volume: 127 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-05 Completed Date: 2004-09-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 155-65 Citation Subset: AIM; IM |
Affiliation:
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Department of Immunology, SIBS, Strathclyde University, Glasgow, United Kingdom. catherine.lawrence@strath |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chymases Host-Parasite Interactions / immunology Intestinal Diseases, Parasitic / etiology, immunology* Mice Serine Endopeptidases / adverse effects, immunology* Th2 Cells / immunology Trichinella spiralis / immunology* Trichinellosis / complications, immunology* |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.39/Chymases; EC 3.4.21.39/Mcpt1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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