Document Detail


Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse.
MedLine Citation:
PMID:  15236182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND & AIMS: The relationship between intestinal pathology and immune expulsion of gastrointestinal nematodes remains controversial. Immune expulsion of gastrointestinal helminth parasites is usually associated with Th2 responses, but the effector mechanisms directly responsible for parasite loss have not been elucidated. Mast cell hyperplasia is a hallmark of infection with gastrointestinal nematodes, in particular Trichinella spiralis. Although the precise mechanism by which mast cells induce expulsion of these parasites has not been elucidated, it has been proposed that mast cell mediators, including cytokines and granule chymases, act to create an environment inhospitable to the parasite, part of this being the induction of intestinal inflammation. Therefore, the aims of this study were to dissect the role of mast cells and mast cell proteases in the induction of parasite-induced enteropathy. METHODS: Mast cell-deficient W/Wv and mouse mast cell protease-1 (mMCP-1)-deficient mice were infected with T. spiralis, and parasite expulsion, enteropathy, and Th2 responses were determined. RESULTS: Expulsion of the parasite was delayed in both strains of mice compared with wild-type controls; additionally, in both cases, the enteropathy was significantly ameliorated. Although Th2 responses were significantly reduced in mast cell-deficient W/Wv mice, those from mMCP-1-deficient mice were similar to wild-type mice. Additionally, levels of TNF-alpha and nitric oxide were significantly reduced in both W/Wv and mMCP-1 deficient mice. CONCLUSIONS: These results imply that mast cells may contribute to the induction of protective Th2 responses and, importantly, that the intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1.
Authors:
Catherine E Lawrence; Yvonne Y W Paterson; Steven H Wright; Pamela A Knight; Hugh R P Miller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastroenterology     Volume:  127     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-05     Completed Date:  2004-09-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  155-65     Citation Subset:  AIM; IM    
Affiliation:
Department of Immunology, SIBS, Strathclyde University, Glasgow, United Kingdom. catherine.lawrence@strath
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MeSH Terms
Descriptor/Qualifier:
Animals
Chymases
Host-Parasite Interactions / immunology
Intestinal Diseases, Parasitic / etiology,  immunology*
Mice
Serine Endopeptidases / adverse effects,  immunology*
Th2 Cells / immunology
Trichinella spiralis / immunology*
Trichinellosis / complications,  immunology*
Chemical
Reg. No./Substance:
EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.39/Chymases; EC 3.4.21.39/Mcpt1 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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