Document Detail


Mouse isolated perfused heart: characteristics and cautions.
MedLine Citation:
PMID:  14678252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Owing to the considerable potential for manipulating the murine genome and, as a consequence, the increasing availability of genetically modified models of cardiovascular diseases, the mouse is fast becoming a cornerstone of animal research. However, progress in the use of various murine preparations is hampered by the lack of facilities and skills for the adequate physiological assessment of genetically modified mice. 2. We have attempted to address this problem by refining and characterizing a mouse isolated heart preparation that was originally developed for use with larger hearts. 3. We used the isolated buffer-perfused Langendorff preparation (perfused at constant flow or constant pressure) to characterize: (i) the frequency-response characteristics; (ii) heart isolation conditions; (iii) perfusion chamber conditions; (iv) temperature-function relationships; (v) stability over extended periods of perfusion; (vi) perfusate calcium-function relationships; (vii) pressure-volume relationships; (viii) pressure-rate relationships; and (ix) flow-function relationships.
Authors:
Fiona J Sutherland; Michael J Shattock; Kathryn E Baker; David J Hearse
Publication Detail:
Type:  In Vitro; Journal Article; Review    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  30     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-17     Completed Date:  2004-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  867-78     Citation Subset:  IM    
Affiliation:
Cardiovascular Research, The Rayne Institute, St Thomas' Hospital, London, UK. fiona.sutherland@kcl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Heart / physiology*
Heart Rate / physiology
Mice
Models, Animal*
Myocardial Reperfusion
Perfusion / instrumentation*,  methods*

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