| Mouse isolated perfused heart: characteristics and cautions. | |
| | |
MedLine Citation:
|
PMID: 14678252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
1. Owing to the considerable potential for manipulating the murine genome and, as a consequence, the increasing availability of genetically modified models of cardiovascular diseases, the mouse is fast becoming a cornerstone of animal research. However, progress in the use of various murine preparations is hampered by the lack of facilities and skills for the adequate physiological assessment of genetically modified mice. 2. We have attempted to address this problem by refining and characterizing a mouse isolated heart preparation that was originally developed for use with larger hearts. 3. We used the isolated buffer-perfused Langendorff preparation (perfused at constant flow or constant pressure) to characterize: (i) the frequency-response characteristics; (ii) heart isolation conditions; (iii) perfusion chamber conditions; (iv) temperature-function relationships; (v) stability over extended periods of perfusion; (vi) perfusate calcium-function relationships; (vii) pressure-volume relationships; (viii) pressure-rate relationships; and (ix) flow-function relationships. |
| | |
Authors:
|
Fiona J Sutherland; Michael J Shattock; Kathryn E Baker; David J Hearse |
Publication Detail:
|
Type: In Vitro; Journal Article; Review |
Journal Detail:
|
Title: Clinical and experimental pharmacology & physiology Volume: 30 ISSN: 0305-1870 ISO Abbreviation: Clin. Exp. Pharmacol. Physiol. Publication Date: 2003 Nov |
Date Detail:
|
Created Date: 2003-12-17 Completed Date: 2004-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0425076 Medline TA: Clin Exp Pharmacol Physiol Country: Australia |
Other Details:
|
Languages: eng Pagination: 867-78 Citation Subset: IM |
Affiliation:
|
Cardiovascular Research, The Rayne Institute, St Thomas' Hospital, London, UK. fiona.sutherland@kcl.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Heart / physiology* Heart Rate / physiology Mice Models, Animal* Myocardial Reperfusion Perfusion / instrumentation*, methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Redox-dependent signalling by angiotensin II and vascular remodelling in hypertension.
Next Document: Responses to ischaemia and reperfusion in the mouse isolated perfused heart and the phenomenon of 'c...