| Mouse ghrelin-O-acyltransferase (GOAT) plays a critical role in bile acid reabsorption. | |
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MedLine Citation:
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PMID: 21965605 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ghrelin is a unique peptide gut hormone that requires post-translational modification to stimulate both feeding and growth hormone release. Ghrelin O-acyltransferase (GOAT) was identified as a specific acyl-transferase for ghrelin, and recent genetic deletion studies of the Goat gene (Goat(-/-)) uncovered the role of ghrelin in the regulation of glucose homeostasis. To further understand the physiological functions of the GOAT/ghrelin system, we have conducted a metabolomic and microarray profile of Goat-null mice, as well as determined Goat expression in different tissues using the lacZ reporter gene. Serum metabolite profile analysis revealed that Goat(-/-) mice exhibited increased secondary bile acids >2.5-fold. This was attributed to increased mRNA and protein expression of the ileal sodium-dependent bile acid transporter (ISBT) in the intestinal and biliary tract. Increased expression of additional solute carrier proteins, including Slc5a12 (>10-fold) were also detected in the small intestine and bile duct. Goat staining was consistently observed in the pituitary glands, stomach, and intestines, and to a lesser extent in the gallbladder and pancreatic duct. This is the first report that the GOAT/ghrelin system regulates bile acid metabolism, and these findings suggest a novel function of GOAT in the regulation of intestinal bile acid reabsorption.-Kang, K., Schmahl, J., Lee, J. -M., Garcia, K., Patil, K., Chen, A., Keene, M., Murphy, A., Sleeman, M. W. Mouse ghrelin-O-acyltransferase (GOAT) plays a critical role in bile acid reabsorption. |
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Authors:
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Kihwa Kang; Jennifer Schmahl; Jong-Min Lee; Karen Garcia; Ketan Patil; Amelia Chen; Michelle Keene; Andrew Murphy; Mark W Sleeman |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-29 |
Journal Detail:
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Title: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: - ISSN: 1530-6860 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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*Regeneron Pharmaceuticals, Tarrytown, New York, USA; and. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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