Document Detail


Mouse exo utero development system: protocol and troubleshooting.
MedLine Citation:
PMID:  18983587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The exo utero development system allows us to manipulate or operate on live embryos of mice or rats at mid- to late gestation stages, from late organogenetic to histogenetic periods, and keep them alive in situ until the analysis of their effects at a desired time point. We can examine the effects of injecting bioactive molecules or cells into targeted parts of a live embryo, destroying specific embryonic regions, or performing fetal surgery. This system is far simpler and more time- and cost-effective for in vivo functional analyses than establishing genetically modified mouse lines and provides a fine-tuned experimental design for developmental scientists. To promote use of the mouse exo utero development system, we elaborate on the technical procedures, discuss critical points for troubleshooting the system, and illustrate some apparatuses essential for fetal microinjection.
Authors:
Makiko Yamada; Toshihisa Hatta; Hiroki Otani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Congenital anomalies     Volume:  48     ISSN:  0914-3505     ISO Abbreviation:  Congenit Anom (Kyoto)     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2009-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306292     Medline TA:  Congenit Anom (Kyoto)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  183-7     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Developmental Biology / instrumentation,  methods*
Embryo Research*
Embryo, Mammalian*
Female
Mice
Microinjections / instrumentation,  methods
Pregnancy
Pregnancy, Animal*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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