| Mouse exo utero development system: protocol and troubleshooting. | |
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MedLine Citation:
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PMID: 18983587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The exo utero development system allows us to manipulate or operate on live embryos of mice or rats at mid- to late gestation stages, from late organogenetic to histogenetic periods, and keep them alive in situ until the analysis of their effects at a desired time point. We can examine the effects of injecting bioactive molecules or cells into targeted parts of a live embryo, destroying specific embryonic regions, or performing fetal surgery. This system is far simpler and more time- and cost-effective for in vivo functional analyses than establishing genetically modified mouse lines and provides a fine-tuned experimental design for developmental scientists. To promote use of the mouse exo utero development system, we elaborate on the technical procedures, discuss critical points for troubleshooting the system, and illustrate some apparatuses essential for fetal microinjection. |
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Authors:
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Makiko Yamada; Toshihisa Hatta; Hiroki Otani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Congenital anomalies Volume: 48 ISSN: 0914-3505 ISO Abbreviation: Congenit Anom (Kyoto) Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-05 Completed Date: 2009-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306292 Medline TA: Congenit Anom (Kyoto) Country: Japan |
Other Details:
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Languages: eng Pagination: 183-7 Citation Subset: IM |
Affiliation:
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Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Developmental Biology / instrumentation, methods* Embryo Research* Embryo, Mammalian* Female Mice Microinjections / instrumentation, methods Pregnancy Pregnancy, Animal* |
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