Document Detail


Mouse cloning and somatic cell reprogramming using electrofused blastomeres.
MedLine Citation:
PMID:  21187860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte availability. However, to date only zygotes have been successfully used as recipients of nuclei from terminally differentiated somatic cell donors leading to ES cell lines. In fertility clinics, embryos of advanced embryonic stages are usually stored for future use, but their ability to support the derivation of ES cell lines via somatic nuclear transfer has not yet been proved. Here, we report that two-cell stage electrofused mouse embryos, arrested in mitosis, can support developmental reprogramming of nuclei from donor cells ranging from blastomeres to somatic cells. Live, full-term cloned pups from embryonic donors, as well as pluripotent ES cell lines from embryonic or somatic donors, were successfully generated from these reconstructed embryos. Advanced stage pre-implantation embryos were unable to develop normally to term after electrofusion and transfer of a somatic cell nucleus, indicating that discarded pre-implantation human embryos could be an important resource for research that minimizes the ethical concerns for human therapeutic cloning. Our approach provides an attractive and practical alternative to therapeutic cloning using donated oocytes for the generation of patient-specific human ES cell lines.
Authors:
Amjad Riaz; Xiaoyang Zhao; Xiangpeng Dai; Wei Li; Lei Liu; Haifeng Wan; Yang Yu; Liu Wang; Qi Zhou
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-28
Journal Detail:
Title:  Cell research     Volume:  21     ISSN:  1748-7838     ISO Abbreviation:  Cell Res.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-30     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  770-8     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1st Beichen West Road, Beijing 100101, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastomeres / cytology*,  metabolism
Cell Fusion / methods*
Cell Line
Chromosomes, Mammalian / metabolism
Cloning, Organism*
Cytoplasm / metabolism
Electroporation / methods*
Embryo, Mammalian / cytology
Embryonic Stem Cells / cytology,  metabolism
Fibroblasts / cytology
Humans
Mice
Mitosis
Nuclear Reprogramming*
Octamer Transcription Factor-3 / metabolism
Pluripotent Stem Cells / cytology,  metabolism
Tetraploidy
Chemical
Reg. No./Substance:
0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse
Comments/Corrections

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