| Mouse breathalyzer. | |
| | |
MedLine Citation:
|
PMID: 18537938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The development of a relatively simple, noninvasive method for estimating blood ethanol concentrations in mice will be useful in behavioral studies related to alcoholism. This study validated such a method. METHODS: The apparatus consists of a body chamber fitted with a head stock through which the mouse head protrudes. This was fitted against a water-jacketed head-space chamber surrounding the mouse's head. Rebreathed air maintained at 37 degrees C in the head-space chamber was removed using a peristaltic pump and loaded into a 1-ml injection loop. Ethanol in the sample was quantified using gas chromatography. To validate this method, ethanol levels in breath samples were compared against those in tail blood samples collected immediately after the breath samples. Breath samples were collected at 5, 10, 20, 40, 80, 120, and 160 minutes after ethanol (0.4, 0.8, 1.2, 1.6, 2.4, and 3.2 g/kg) was administered to male C57BL/6J mice. RESULTS: Breath and blood ethanol levels were well correlated (r(2) = 0.96) across time points on the descending ethanol-time curve at doses below 2.4 g/kg. Correlation for these doses on the ascending portion of the curve had greater variance, but was still well correlated (r(2) = 0.92). CONCLUSIONS: The mouse breathalyzer is an accurate, convenient, noninvasive and well-tolerated method for estimating blood ethanol concentrations in mice across a range of behaviorally relevant concentrations below 2.4 g/kg, especially on the descending limb of the ethanol-time curve. Although this procedure requires a gas chromatograph in the animal facility, the ability to estimate ethanol concentrations quickly and easily will be especially useful in behavioral studies where repeated blood sampling is not feasible. |
| | |
Authors:
|
Brett C Ginsburg; Martin A Javors; Gregory Friesenhahn; Michael Frontz; Gerardo Martinez; Tim Hite; Richard J Lamb |
Related Documents
:
|
8053118 - Pharmacokinetics of sodium humate in chickens. 12460348 - Validation of the ethanol breath test and on-table weighing to measure irrigating fluid... 9369998 - Subclinical neurological and neurovascular deficits in cocaine dependence. gender and p... 20015248 - Lack of effect on blood alcohol level of swabbing venepuncture sites with 70% isopropyl... 6796658 - Osmotic blood-brain barrier disruption in the posterior fossa of the dog. 8081648 - Helicobacter pylori: molecular basis for host recognition and bacterial adherence. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Validation Studies |
Journal Detail:
|
Title: Alcoholism, clinical and experimental research Volume: 32 ISSN: 1530-0277 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 2008 Jul |
Date Detail:
|
Created Date: 2008-07-11 Completed Date: 2008-10-07 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: England |
Other Details:
|
Languages: eng Pagination: 1181-5 Citation Subset: IM |
Affiliation:
|
Department of Psychiatry, The University of Texas Health Science Center at San Antonio, Texas 78229, USA. ginsburg@uthscsa.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Breath Tests / instrumentation, methods* Ethanol / analysis*, blood Mice Mice, Inbred C57BL |
| Grant Support | |
ID/Acronym/Agency:
|
AA12337/AA/NIAAA NIH HHS; R01 AA012337-07/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
64-17-5/Ethanol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Detrimental effect of preservatives in eyedrops: implications for the treatment of glaucoma.
Next Document: Effects of opioid receptor gene variation on targeted nalmefene treatment in heavy drinkers.