Document Detail


Mouse Y-specific repeats isolated by whole chromosome representational difference analysis.
MedLine Citation:
PMID:  8812464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Representational difference analysis (RDA) was used to generate Y-specific probes by enriching for and cloning the differences between the male (XY) and the female (XX) C57BL/6J mouse genomes. Characterization of 35 clones revealed 12 families related by sequence similarity. One clone from each family was chosen for detailed analysis by Southern blot hybridization, polymerase chain reaction (PCR) on normal and aberrant genomes (Sxr), and fluorescence in situ hybridization. From one difference product we have characterized 12 Y-specific probes for hybridization, created seven male-specific PCR assays, mapped all repeat families, and identified one repeat with a distinct XY homology. We report the first cloning of a Y-specific long interspersed repeat element (LINE) fragment. In total, RDA has identified six novel Y Chromosome repeat families and allowed us to extend the characterization of six known Y repeats. We conclude that this novel use of RDA for whole chromosome subtraction successfully enriches chromosome-specific sequences and is suitable for the rapid generation of new Y Chromosome-specific probes.
Authors:
A Navin; R Prekeris; N A Lisitsyn; M M Sonti; D A Grieco; S Narayanswami; E S Lander; E M Simpson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  36     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-01-23     Completed Date:  1997-01-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-53     Citation Subset:  IM    
Affiliation:
The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine, 04609, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U43974;  U43975;  U43976;  U43977;  U43978;  U43979;  U43980;  U43981;  U43982;  U43983;  U43984;  U43985
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
DNA Probes*
Female
In Situ Hybridization, Fluorescence
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Polymerase Chain Reaction
Y Chromosome*
Grant Support
ID/Acronym/Agency:
CA34196/CA/NCI NIH HHS; HG00098/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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