| Mouse U14 snRNA is a processed intron of the cognate hsc70 heat shock pre-messenger RNA. | |
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MedLine Citation:
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PMID: 1282084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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U14 snRNA is a small nucleolar RNA species essential for eukaryotic pre-rRNA processing. We have previously shown that the mouse U14 snRNA genes are positioned within introns 5, 6, and 8 on the coding strand of the constitutively expressed cognate hsc70 heat shock gene. This genomic organization suggested the possibility that U14 snRNAs are transcribed as part of the hsc70 pre-mRNA and then excised from the intron to yield mature U14 snRNA species. To test this hypothesis directly, we have microinjected Xenopus oocytes with hsc70 pre-mRNA transcripts possessing intron 5 and the encoded U14 snRNA sequence. Processing results demonstrate that, in addition to the splicing of upstream and downstream exons, a mature 87 nt U14 snRNA is excised from the intron. Accurate excision of U14 snRNA from hsc70 intron 5 can occur in the absence of splicing. These results demonstrate a biosynthetic pathway for an snRNA species and provide a novel example of a eukaryotic pre-mRNA intron that is processed to produce a stable, biologically functional RNA species. |
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Authors:
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R D Leverette; M T Andrews; E S Maxwell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 71 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-02-03 Completed Date: 1993-02-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1215-21 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, North Carolina State University, Raleigh 27695. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Heat-Shock Proteins / genetics* Introns Mice Molecular Sequence Data RNA / biosynthesis* RNA Editing* |
| Grant Support | |
ID/Acronym/Agency:
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HD-24673/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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