Document Detail

Mouse U14 snRNA is a processed intron of the cognate hsc70 heat shock pre-messenger RNA.
MedLine Citation:
PMID:  1282084     Owner:  NLM     Status:  MEDLINE    
U14 snRNA is a small nucleolar RNA species essential for eukaryotic pre-rRNA processing. We have previously shown that the mouse U14 snRNA genes are positioned within introns 5, 6, and 8 on the coding strand of the constitutively expressed cognate hsc70 heat shock gene. This genomic organization suggested the possibility that U14 snRNAs are transcribed as part of the hsc70 pre-mRNA and then excised from the intron to yield mature U14 snRNA species. To test this hypothesis directly, we have microinjected Xenopus oocytes with hsc70 pre-mRNA transcripts possessing intron 5 and the encoded U14 snRNA sequence. Processing results demonstrate that, in addition to the splicing of upstream and downstream exons, a mature 87 nt U14 snRNA is excised from the intron. Accurate excision of U14 snRNA from hsc70 intron 5 can occur in the absence of splicing. These results demonstrate a biosynthetic pathway for an snRNA species and provide a novel example of a eukaryotic pre-mRNA intron that is processed to produce a stable, biologically functional RNA species.
R D Leverette; M T Andrews; E S Maxwell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  71     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-03     Completed Date:  1993-02-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1215-21     Citation Subset:  IM    
Department of Biochemistry, North Carolina State University, Raleigh 27695.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Heat-Shock Proteins / genetics*
Molecular Sequence Data
RNA / biosynthesis*
RNA Editing*
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins; 63231-63-0/RNA

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