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A Mouse Model of Reverse Cardiac Remodelling Following Banding-Debanding of the Ascending Aorta.
MedLine Citation:
PMID:  21974781     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aim:  Myocardial remodelling during pressure overload might contribute to development of heart failure. Reverse remodelling normally occurs following aortic valve replacement for aortic stenosis, however, the details and regulatory mechanisms of reverse remodelling remain unknown. Thus, an experimental model of reverse remodelling would allow for studies of this process. Although models of aortic banding are widely used, only few reports of debanding models exist. The aim of the present study was to establish a banding-debanding model in the mouse with repetitive careful hemodynamic evaluation by high resolution echocardiography. Methods:  C57Bl/6 mice were subjected to ascending aortic banding and subsequent debanding. Cardiac geometry and function were evaluated by echocardiography, left ventricular myocardium was analyzed by histology and QPCR. Results:  The degree of aortic banding was controlled by non-invasive estimation of the gradient and we found a close correlation between left ventricular mass estimated by echocardiography and weight at sacrifice. Aortic banding led to left ventricular hypertrophy, fibrosis and expression of fetal genes, indicating myocardial remodelling. Echocardiography revealed concentric left ventricular remodelling and myocardial dysfunction. Following debanding, performed via a different incision, there was rapid regression of left ventricular weight and normalization of both cardiac geometry and function by 14 days. Conclusions:  We have established a reproducible and carefully characterized mouse model of reverse remodelling by banding and debanding of the ascending aorta. Such a model might contribute to increased understanding of the reversibility of cardiac pathology, which in turn might give rise to new strategies in heart failure treatment.
Authors:
Johannes L Bjørnstad; Biljana Skrbic; Ivar Sjaastad; Sigrid Bjørnstad; Geir Christensen; Theis Tønnessen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-4
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Scandinavian Physiological Society.
Affiliation:
Department of Cardiothoracic Surgery, Oslo University Hospital Ullevål Faculty of Medicine, University of Oslo Institute for Experimental Medical Research, Oslo University Hospital Ullevål Department of Cardiology, Oslo University Hospital Ullevål Center for Heart Failure Research, University of Oslo Department of Pathology, Oslo University Hospital Ullevål, Oslo, Norway.
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