Document Detail

Mouse C2 myoblast cells resist HVJ (Sendai virus)-mediated cell fusion in the proliferating stage but become capable of fusion after differentiation.
MedLine Citation:
PMID:  10365439     Owner:  NLM     Status:  MEDLINE    
To investigate the mechanism of myoblast fusion, we attempted to prepare artificial myotubes of mouse C2 myoblast cells using the hemagglutinating virus of Japan (HVJ, Sendai virus). Proliferating C2 cells showed strong resistance to HVJ-mediated cell fusion and remained morphologically unchanged even though massive numbers of virions adsorbed onto their surface. They showed no membrane disruption, which occurs in the early stage of cell fusion induced by HVJ. These observations suggest that proliferating C2 cells are resistant to HVJ-mediated cell fusion. However, upon induction of differentiation, C2 cells gradually became capable of fusion induced by HVJ and then even generated heterokaryons with Ehrlich ascites tumor cells. When differentiated C2 cells that had become fusion-sensitive were treated with HVJ in the presence of EDTA, they did not fuse but degenerated, suggesting that their cell membranes were transiently disrupted by interaction with HVJ. These results suggest that the cell membranes of myoblasts change to a fusion-capable state during the process of differentiation.
E Hirayama; M Nakanishi; N Honda; J Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  64     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-07-09     Completed Date:  1999-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  213-23     Citation Subset:  IM    
Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University 1, Japan.
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MeSH Terms
Avian Sarcoma Viruses
Carcinoma, Ehrlich Tumor / pathology,  physiopathology
Cell Differentiation
Cell Division
Cell Fusion / physiology*
Cell Line
Cell Line, Transformed
Chick Embryo
Edetic Acid / pharmacology
Muscle, Skeletal / cytology*,  drug effects,  physiology
Respirovirus / physiology*
Tumor Cells, Cultured
Reg. No./Substance:
60-00-4/Edetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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