Document Detail


Mouse 3T3 cell filtrability correlating with concanavalin A agglutinability.
MedLine Citation:
PMID:  6509087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a previous study of the dextran gel sphere model system, a possible correlation between cell deformability and agglutinability by concanavalin A was indicated. Cell deformability was evaluated as filtrability, using polycarbonate membrane filtration. With 25-mm diameter filters and 5-ml cell suspensions at (0.8-16) X 10(5) cells/ml, the filtrability at a given filter pore size was highly reproducible and was not affected by variations in cell population, viability, washings of cells retained on filter, or temperature. The filtrability of EDTA-dissociated 3T3 cells through 12-micron pore size filter was 8%, and a suspension of 10(6) cells/ml was not agglutinated by 600 micrograms concanavalin A. The filtrability of trypsin-dissociated 3T3 cells was 95%, and these cells were agglutinated by 200 micrograms of the lectin. EDTA-dissociated SV-3T3 cells had a filtrability of 73% and were also highly agglutinable. Formalin fixation reduced the high filtrability to 6%, and also abolished the agglutinability. As a further test of the correlation, trypsin-dissociated 3T3 cells were admixed with the fixed cells. The agglutinability varied with the proportions of the two cell components, and the admixtures could be separated according to filtrability into the original components with distinctly different agglutinability. Furthermore, 25% of a random population of EDTA-dissociated SV-3T3 cells retained by the filter were found to be non-agglutinable. The separated SV-3T3 cell fractions could also form admixtures of different agglutinability. It is concluded that the agglutinability of mouse 3T3 and SV-3T3 cells by concanavalin A can be correlated with and predicted by cell filtrability.
Authors:
P Y Wang; J H Youson; T T Drakos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  802     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-11     Completed Date:  1985-02-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  467-76     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination / drug effects
Animals
Cell Survival
Cold Temperature
Concanavalin A / pharmacology*
Fibroblasts / cytology*,  drug effects
Filtration
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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