| Motor apparatus in human spermatozoa that lack central pair microtubules. | |
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MedLine Citation:
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PMID: 12412048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electron microscopic examination of the spermatozoa from a man suffering from asthenozoospermia (poor or low sperm motility) showed that approximately 92% of the sperm flagella lacked central pair microtubules but possessed dynein arms and radial spokes while a small percentage of the spermatozoa had complete flagella. The characteristics of the motor apparatus of the spermatozoa and the effects of caffeine on the sperm motility were examined, as were the reactivation of demembranated spermatozoa and the sliding of doublet microtubules. Almost all spermatozoa were immotile in a Tyrode solution while only a small percentage of spermatozoa showed slow forward movement or feeble flagellar vibration, whereas addition of caffeine to the sperm suspension induced forward swimming of approximately half of the spermatozoa. The reactivation of demembranated spermatozoa with MgATP(2-) could not succeed because of disintegration of the demembranated flagella. However, when the demembranated spermatozoa were exposed to MgATP(2-) and then treated with elastase, the microtubular doublets of approximately half the number of the flagella slid from the end or middle of the flagella. These results suggest that the motor apparatus in the sperm flagella that lack the central pair microtubules is functionally assembled and intrinsically capable of undergoing flagellar movement but not strong enough to beat normally. |
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Authors:
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Sumio Ishijima; Teruaki Iwamoto; Shiari Nozawa; Kazuhiko Matsushita |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular reproduction and development Volume: 63 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-04 Completed Date: 2003-12-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
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Languages: eng Pagination: 459-63 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley-Liss, Inc. |
Affiliation:
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Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo, Japan. sishijim@bio.titech.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Caffeine / pharmacology Humans Male Microscopy, Electron Microscopy, Phase-Contrast Microtubules / drug effects, metabolism*, ultrastructure Sperm Motility* / drug effects Spermatozoa / cytology*, drug effects, physiology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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58-08-2/Caffeine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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