Document Detail


Motoneurons regulate myoblast proliferation and patterning in Drosophila.
MedLine Citation:
PMID:  15617689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Motoneurons directly influence the differentiation of muscle fibers, regulating features such as muscle fiber type and receptor development. Less well understood is whether motoneurons direct earlier events, such as the patterning of the musculature. In Drosophila, the denervation of indirect flight muscles results in a diminished myoblast population and smaller or missing muscle fibers. We have examined whether the neuron-dependent control of myoblast number is due to regulation of cell division, motoneuron-dependent apoptosis, or nerve-dependent localization and migration of myoblasts. We found that denervation resulted in a reduced rate of cell division, as revealed by BrDU incorporation. There was no change in the frequency of apoptotic myoblasts following denervation. Using time lapse imaging of GFP-expressing myoblasts in vivo in pupae, we observed that despite denervation, the migration and localization of myoblasts remained unchanged. In addition to reducing myoblast proliferation, denervation also altered the segregation of myoblasts into the de novo arising dorso-ventral muscles (DVMs). To address this effect on muscle patterning, we examined the expression of the founder-cell marker Dumbfounded/Kirre (Duf) in imaginal pioneer cells. We show that there is a strong correspondence between cells that express Dumbfounded/Kirre and the number of DVM fibers, consistent with a role for these cells in establishing adult muscles. In the absence of innervation the Duf-positive cells are no longer detected, and muscle patterning is severely disrupted. Our results support a model where specialized founder cells prefigure the adult muscle fibers under the control of the nervous system.
Authors:
Joyce J Fernandes; Haig Keshishian
Related Documents :
12757709 - Il-4 acts as a myoblast recruitment factor during mammalian muscle growth.
7048849 - Immuno-histochemical localization of myoglobin in human muscle tissue and embryonal and...
15261649 - Integrating transcriptional and signalling networks during muscle development.
17213899 - Molecular regulation of myogenic progenitor populations.
10603359 - The role of lbx1 in migration of muscle precursor cells.
16144439 - Angiogenic gene-modified muscle cells for enhancement of tissue formation.
8268759 - Immunogold ultrastructural localization of calpastatin, the calpain inhibitor, in rabbi...
17475139 - The relationship between ankle, hindfoot, and forefoot position and posterior tibial mu...
24336879 - Enhancement of satellite cell differentiation and functional recovery in injured skelet...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  277     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-24     Completed Date:  2005-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  493-505     Citation Subset:  IM    
Affiliation:
Zoology Department, 250 Pearson Hall, Miami University, Oxford, OH 45056, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning*
Bromodeoxyuridine
Cell Proliferation*
Drosophila / embryology*
Drosophila Proteins / metabolism
Epigenesis, Genetic*
Fluorescence
Green Fluorescent Proteins
Immunohistochemistry
Membrane Proteins / metabolism
Microscopy, Confocal
Models, Biological
Morphogenesis
Motor Neurons / physiology*
Muscle Proteins / metabolism
Muscles / embryology*
Myoblasts / cytology*
Grant Support
ID/Acronym/Agency:
1R15MH067622-01/MH/NIMH NIH HHS; R01 NS31651/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Membrane Proteins; 0/Muscle Proteins; 0/enhanced green fluorescent protein; 0/kin of irre protein, Drosophila; 147336-22-9/Green Fluorescent Proteins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Xenopus p21-activated kinase 5 regulates blastomeres' adhesive properties during convergent extensio...
Next Document:  Functional role of a novel ternary complex comprising SRF and CREB in expression of Krox-20 in early...