Document Detail

Motility and invasive potency of murine T-lymphoma cells: effect of microtubule inhibitors.
MedLine Citation:
PMID:  7910504     Owner:  NLM     Status:  MEDLINE    
ESb and BW-O-Li1 are T-lymphoma cell lines that form metastases in various organs after injection into syngeneic mice. In vitro, both cell lines invade through a fibroblastic monolayer, but ESb cells do so much slower than BW-O-Li1. By the use of Fourier analysis of cell outlines, we can relate this difference in invasiveness to a difference in cell motility: ESb cells do not perform any conspicuous shape change, whereas BW-O-Li1 cells are actively protruding and retracting large pseudopodia. However, the low-motile ESb cells become as motile and deformable as BW-O-Li1 cells when they have eventually invaded under a fibroblastic monolayer. This indicates that ESb cells do have inherent capability for shape change. Treatment of ESb cells with the microtubule disrupting agent nocodazole concomitantly increases their shape change intensity, and their invasion rate through fibroblast monolayers. On the contrary, the microtubule stabilizing drug taxol inhibits both motility and invasion of BW-O-Li1 cells. Our observations suggest that the microtubule network can repress invasion-bound motility of lymphoid cells.
H Verschueren; J Dewit; J De Braekeleer; V Schirrmacher; P De Baetselier
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell biology international     Volume:  18     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-06-17     Completed Date:  1994-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
Pasteur Instituut van Brabant, Brussels, Belgium.
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MeSH Terms
Cell Movement
Fourier Analysis
Leukemia L5178 / pathology
Lymphoma, T-Cell / pathology*
Mice, Inbred DBA
Microtubules / drug effects,  physiology
Neoplasm Invasiveness / pathology*
Nocodazole / pharmacology
Paclitaxel / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
31430-18-9/Nocodazole; 33069-62-4/Paclitaxel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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