| Motility and invasive potency of murine T-lymphoma cells: effect of microtubule inhibitors. | |
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MedLine Citation:
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PMID: 7910504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ESb and BW-O-Li1 are T-lymphoma cell lines that form metastases in various organs after injection into syngeneic mice. In vitro, both cell lines invade through a fibroblastic monolayer, but ESb cells do so much slower than BW-O-Li1. By the use of Fourier analysis of cell outlines, we can relate this difference in invasiveness to a difference in cell motility: ESb cells do not perform any conspicuous shape change, whereas BW-O-Li1 cells are actively protruding and retracting large pseudopodia. However, the low-motile ESb cells become as motile and deformable as BW-O-Li1 cells when they have eventually invaded under a fibroblastic monolayer. This indicates that ESb cells do have inherent capability for shape change. Treatment of ESb cells with the microtubule disrupting agent nocodazole concomitantly increases their shape change intensity, and their invasion rate through fibroblast monolayers. On the contrary, the microtubule stabilizing drug taxol inhibits both motility and invasion of BW-O-Li1 cells. Our observations suggest that the microtubule network can repress invasion-bound motility of lymphoid cells. |
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Authors:
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H Verschueren; J Dewit; J De Braekeleer; V Schirrmacher; P De Baetselier |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cell biology international Volume: 18 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-06-17 Completed Date: 1994-06-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 11-9 Citation Subset: IM |
Affiliation:
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Pasteur Instituut van Brabant, Brussels, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Fibroblasts Fourier Analysis Leukemia L5178 / pathology Lymphoma, T-Cell / pathology* Mice Mice, Inbred DBA Microtubules / drug effects, physiology Neoplasm Invasiveness / pathology* Nocodazole / pharmacology Paclitaxel / pharmacology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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31430-18-9/Nocodazole; 33069-62-4/Paclitaxel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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