Document Detail


Motility and adhesive properties of high- and low-metastatic murine neoplastic cells.
MedLine Citation:
PMID:  6692379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Randomly chosen clones of the murine K-1735 melanoma tumor were used by Raz and Geiger to address the question of whether variations in actin organization in these cells may be related to their lung colonization capability in syngeneic hosts (Cancer Res., 42: 5183-5190, 1982). In 14 of 15 clones tested, we found that the degree of actin organization was inversely correlated to their metastatic capability. We have further shown that remarkable variations exist in the adhesive properties and locomotor activity of four K-1735 melanoma cell variants that exhibit distinct metastatic properties. The low-metastatic cell variants displayed large focal adhesion plaques, tightly packed actin bundles, elaborate extracellular networks of fibronectin, and restricted motility. In contrast, the high-metastatic variants were poorly attached with only few distinct actin bundles, were unable to reorganize extracellular fibronectin into cables, and exhibited high motile activity. Electron microscopic examination of local s.c. tumors of the high- and low-metastatic lines indicated that the former formed loose tumor masses with very few intercellular connections, while the low-metastatic line developed into a considerably more compact tumor with numerous intercellular contacts, in line with the in vitro findings. It is proposed that the differences in cellular properties manifested by these cell lines may be related to their metastatic properties. Specifically, the highly metastatic cells of this tumor system may easily detach from the primary tumor mass, form weak and transient connections with surrounding connective tissue, and actively migrate through it. Furthermore, these results point to the close interrelationships between different mechanochemical features in cells, including specific cell adhesiveness, cytoskeletal organization, locomotion, and rearrangement of extracellular fibronectin. The possible nature of these interrelationships is discussed.
Authors:
T Volk; B Geiger; A Raz
Related Documents :
14719109 - Deletion in p16ink4a and loss of p16 expression in human skin primary and metastatic me...
3368969 - Establishment and characterization of a new human melanoma cell line (hu 214) with a hi...
12114539 - Mda-7 (il-24) mediates selective apoptosis in human melanoma cells by inducing the coor...
10922199 - Metastatic melanoma death rates by anatomic site after proton beam irradiation for uvea...
12470139 - Noncontiguous tumor recurrence of posterior uveal melanoma after transscleral local res...
1293169 - Gangliosides of melanoma.
9600129 - Effects of boron neutron capture therapy using borocaptate sodium in combination with a...
20178649 - Pediatric primary central nervous system germ cell tumors of different prognosis groups...
14719109 - Deletion in p16ink4a and loss of p16 expression in human skin primary and metastatic me...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  44     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-03-01     Completed Date:  1984-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  811-24     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / analysis
Animals
Cell Adhesion
Cell Line
Cell Movement
Fibronectins / analysis
Melanoma / pathology*
Mice
Microscopy, Electron
Neoplasm Metastasis / pathology
Time Factors
Chemical
Reg. No./Substance:
0/Actins; 0/Fibronectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Carbohydrate chain analysis by lectin binding to electrophoretically separated glycoproteins from mu...
Next Document:  Thymidine as a kinetic and biochemical modulator of 1-beta-D-arabinofuranosylcytosine in human acute...