| Motilin stimulates preadipocyte proliferation and differentiation and adipocyte lipid storage. | |
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MedLine Citation:
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PMID: 21771971 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Motilin is a circulating gastrointestinal peptide secreted primarily by duodenal mucosal M cells and recognized for its prokinetic effects on gastrointestinal tissues. Little information is available regarding effects on insulin/glucose homeostasis or adipocyte function. Aim: To evaluate the effects of motilin on adipocyte proliferation, differentiation, lipolysis and macronutrient uptake in adipocytes. Methods: 3T3-L1 cells and primary rat adipocytes were treated acutely and chronically with varying motilin concentrations and effects were compared to vehicle alone (control), set as 100% for all assays. Results: In preadipocytes, motilin stimulated proliferation ((3)H-thymidine incorporation) and mitochondrial activity (up to 141%±10%,p<0.001 and 158%±10%, p<0.001, respectively), in a concentration-dependent manner. Chronic supplementation with motilin during differentiation further increased lipogenesis (Oil red O staining 191±27%, p<0.05) and was associated with an up-regulation of PPAR-γ (148±8%, p<0.01), C/EBPα (142±17%, p<0.05) and Cav3 (166±20%, p<0.05) expression. In mature 3T3-L1 adipocytes motilin increased fatty acid uptake/incorporation (up to 202±12%; p<0.01), glucose uptake (up to 146±9% p<0.05) and decreased net fatty acid release (maximal -31%; p<0.05) without influencing total lipolysis (glycerol release). Similar effects were obtained in primary rat adipocytes. Motilin acutely increased expression of PPAR-γ, CEBPβ, DGAT1 and CD36, while decreasing adiponectin mRNA and secretion. In human adipose tissue, motilin receptor GPR38 correlated with HOMA-IR and GHSR1 (r=0.876, p<0.0001). Motilin binding and fatty acid incorporation into adipocytes were inhibited by antagonists MB10 and [D-lys3]-GRP6 and PI3kinase inhibitor wortmannin. Conclusion: Taken together, these results suggest that motilin may directly influence adipocyte functions by stimulating energy storage. |
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Authors:
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Pierre Miegueu; Katherine Cianflone; Denis Richard; David H St-Pierre |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-19 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: - ISSN: 1522-1555 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Laval University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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