Document Detail

The MotA transcription factor from bacteriophage T4 contains a novel DNA-binding domain: the 'double wing' motif.
MedLine Citation:
PMID:  11918797     Owner:  NLM     Status:  MEDLINE    
MotA is a transcription factor from bacteriophage T4 that helps adapt the host Escherichia coli transcription apparatus to T4 middle promoters. We have determined the crystal structure of the C-terminal DNA-binding domain of MotA (MotCF) to 1.6 A resolution using multiwavelength, anomalous diffraction methods. The structure reveals a novel DNA-binding alpha/beta motif that contains an exposed beta-sheet surface that mediates interactions with the DNA. Independent biochemical experiments have shown that MotCF binds to one surface of a single turn of DNA through interactions in adjacent major and minor grooves. We present a model of the interaction in which beta-ribbons at opposite corners of the six-stranded beta-sheet penetrate the DNA grooves, and call the motif a 'double wing' to emphasize similarities to the 'winged-helix' motif. The model is consistent with data on how MotA functions at middle promoters, and provides an explanation for why MotA can form non-specific multimers on DNA.
Ning Li; E Allen Sickmier; Rongguang Zhang; Andrzej Joachimiak; Stephen W White
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular microbiology     Volume:  43     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-28     Completed Date:  2002-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1079-88     Citation Subset:  IM    
Department of Structural Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
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MeSH Terms
Amino Acid Sequence
Bacteriophage T4 / chemistry*
Crystallography, X-Ray
DNA / metabolism
DNA-Binding Proteins / chemistry*,  metabolism
Models, Molecular
Molecular Sequence Data
Transcription Factors / chemistry*,  metabolism
Viral Proteins / chemistry*,  metabolism
Reg. No./Substance:
0/DNA-Binding Proteins; 0/MotA protein, Enterobacteria phage T4; 0/Transcription Factors; 0/Viral Proteins; 9007-49-2/DNA

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