Document Detail

Mosquito cells infected with Japanese encephalitis virus release slowly-sedimenting hemagglutinin particles in association with intracellular formation of smooth membrane structures.
MedLine Citation:
PMID:  16547419     Owner:  NLM     Status:  MEDLINE    
Arthropod-borne flaviviruses can grow in both arthropod and mammalian cells. Virion morphogenesis, though well studied in mammalian cells, is still unclear in arthropod cells. Here, we compared a mosquito cell line C6/36 and a mammalian cell line Vero in extracellular virus particles and intracellular ultrastructures triggered by infection with Japanese encephalitis virus (JEV). Sedimentation analyses of virion and slowly-sedimenting hemagglutinin (SHA) particles released by infection with the Nakayama strain revealed that C6/36 cells produced higher envelope (E) antigen levels in the SHA than the virion fraction in contrast to Vero cells that showed the opposite pattern. Specific infectivities per ng of E were similar in both cells, whereas specific hemagglutinating activities in the SHA fraction were lower in C6/36 than Vero cells. The precursor membrane protein was less efficiently cleaved to the membrane protein in SHA particles released from C6/36 than Vero cells. Ultrastructural studies showed more remarkable production of smooth membrane structures (SMSs) in C6/36 than in Vero cells. The differences in sedimentation patterns of extracellular virus particles between Nakayama-infected C6/36 and Vero cells were consistently observed in 5 other strains (Beijing P1, Beijing P3, JaTH-160, KE-093 and JaGAr-O1), except for KE-093-infected C6/36 cells which exhibited the Vero-type sedimentation profile under conditions of open cultivation. By electron microscopy, the production of SMSs from KE-093-infected C6/36 cells under open conditions was markedly less than that under closed conditions where the cells exhibited the C6/36-type sedimentation profile. Thus, intracellular SMS formations were associated with extracellular SHA production in JEV-infected mosquito cells.
Tomohiro Ishikawa; Eiji Konishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  50     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-03-20     Completed Date:  2006-07-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  211-23     Citation Subset:  IM    
Department of Health Sciences, Kobe University School of Medicine, Kobe, Hyogo 654-0142, Japan.
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MeSH Terms
Cell Line
Cell Membrane / ultrastructure
Cercopithecus aethiops
Culicidae / ultrastructure*,  virology*
Encephalitis Virus, Japanese / growth & development,  pathogenicity*
Hemagglutinins, Viral / chemistry*,  metabolism*
Microscopy, Electron
Vero Cells
Virion / chemistry,  growth & development*
Reg. No./Substance:
0/Hemagglutinins, Viral

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