| Mos positively regulates Xe-Wee1 to lengthen the first mitotic cell cycle of Xenopus. | |
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MedLine Citation:
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PMID: 10072389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several key developmental events occur in the first mitotic cell cycle of Xenopus; consequently this cycle has two gap phases and is approximately 60-75 min in length. In contrast, embryonic cycles 2-12 consist only of S and M phases and are 30 min in length. Xe-Wee1 and Mos are translated and degraded in a developmentally regulated manner. Significantly, both proteins are present in the first cell cycle. We showed previously that the expression of nondegradable Mos, during early interphase, delays the onset of M phase in the early embryonic cell cycles. Here we report that Xe-Wee1 is required for the Mos-mediated M-phase delay. We find that Xe-Wee1 tyrosine autophosphorylation positively regulates Xe-Wee1 and is only detected in the first 30 min of the first cell cycle. The level and duration of Xe-Wee1 tyrosine phosphorylation is elevated significantly when the first cell cycle is elongated with nondegradable Mos. Importantly, we show that the tyrosine phosphorylation of Xe-Wee1 is required for the Mos-mediated M-phase delay. These findings indicate that Mos positively regulates Xe-Wee1 to generate the G2 phase in the first cell cycle and establish a direct link between the MAPK signal transduction pathway and Wee1 in vertebrates. |
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Authors:
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M S Murakami; T D Copeland; G F Vande Woude |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Genes & development Volume: 13 ISSN: 0890-9369 ISO Abbreviation: Genes Dev. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-04-27 Completed Date: 1999-04-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 620-31 Citation Subset: IM |
Affiliation:
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Advanced Bioscience Laboratories (ABL)-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702 USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Cell Cycle / physiology Cell Cycle Proteins* Cell-Free System G2 Phase Mitosis / physiology* Nuclear Proteins* Phosphorylation Protein-Tyrosine Kinases / genetics, metabolism* Proto-Oncogene Proteins c-mos / genetics, metabolism* Tyrosine / metabolism Up-Regulation Xenopus Xenopus Proteins |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Nuclear Proteins; 0/Xenopus Proteins; 55520-40-6/Tyrosine; EC 2.7.1.112/Wee1A protein, Xenopus; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-mos |
| Comments/Corrections | |
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