| Mortality of human epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells. | |
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MedLine Citation:
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PMID: 12680197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal human epidermal keratinocytes (NHEK) died after 5 days of co-culture with an oral carcinoma cell line (HSC-4). However, the HSC-4 cells did not change in either mitotic character or shape. We analyzed the changes in the mitotic activity and DNA synthetic activity of NHEK in co-culture with the HSC-4 cells. The mitotic index of the NHEK decreased gradually with the presence of human beta-defensin-2 (HBD-2) in the media. However, the mitotic index (MI) of the HSC-4 cells did not change. The shape of the NHEK cells did not change during co-culture. The time changes of the labeling index (LI) measured by BrdU incorporation, and the LI decreased in neither NHEK nor in HSC-4 cells in the presence of HBD-2. Moreover, in co-culture with an anti-HBD-2 antibody, the MI of both NHEK and HSC-4 cells did not change. These results indicate that HBD-2 might not lead to apoptosis or necrosis, but rather to cellular reproductive death in NHEK cells. However, HSC-4 cells might be more tolerant of HBD-2, so that HBD-2 does not cause cellular reproductive death. HBD-2 does not diminish the DNA synthesis rate but decreases the mitotic activity in NHEK cells in vitro. HBD-2 diminishes neither the DNA synthesis rate nor the mitotic activity in HSC-4 cells. |
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Authors:
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Koichi Sawaki; Tomoichiro Yamaai; Nobuyoshi Mizukawa; Tomohitio Yoshimoto; Takaaki Ueno; Makoto Nakano; Toshio Sugahara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anticancer research Volume: 23 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2003 Jan-Feb |
Date Detail:
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Created Date: 2003-04-08 Completed Date: 2003-05-01 Revised Date: 2010-09-17 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 79-84 Citation Subset: IM |
Affiliation:
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Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bromodeoxyuridine
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metabolism Carcinoma, Squamous Cell / pathology* Cell Communication / drug effects, physiology* Cell Death / drug effects, physiology Cell Division / drug effects, physiology Coculture Techniques Culture Media, Conditioned Epidermis / cytology Humans Keratinocytes / cytology*, drug effects Mitosis / drug effects, physiology Mouth Neoplasms / pathology* Tumor Cells, Cultured beta-Defensins / pharmacology*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/DEFB4A protein, human; 0/beta-Defensins; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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