Document Detail

Morphometry of cells and guttae in subjects with normal or guttate endothelium with a contour detection algorithm.
MedLine Citation:
PMID:  16021003     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To develop a semiautomatic method to analyze morphology of cells and guttae in corneal endothelium. METHODS: Specular endothelial pictures from 42 and 21 subjects with healthy and guttate corneas, respectively, were analyzed independently by two observers with cell contour-extracting routines. One observer also analyzed healthy endothelia with the Corner method (Bambi). Differences between observers and between methods in mean cell area (MCA), coefficient of variation (CV), and percentage of cells with five, six, or seven sides were tested for significance with paired t tests. The Contour analysis of pictures with guttae included their mean area. RESULTS: There were no significant differences in MCA, CV, or the percentage of cells with five, six, or seven sides between the measurements obtained on repeated analysis by the same observer or on a second analysis performed by a different observer with the Contour method. However, the differences between the Contour and Bambi methods were statistically significant for MCA (337.5 +/- 37.7 vs. 327.7 +/- 36.5), CV (0.32 +/- 0.05 vs. 0.30 +/- 0.05), and percentage of cells with six and seven sides, but not for the percentage of five-sided cells. In subjects with guttata, the MCA was 561 +/- 170 microm, and the mean area of guttae was 1,538 +/- 849 microm. CONCLUSIONS: This detection algorithm is repeatable and reproducible, and it generates a cell border overlay useful in analyzing the morphology of cells and guttae. The analysis of corneal guttae could become a useful follow-up procedure to discriminate between patients with corneal guttata and Fuchs dystrophy.
Claude J Giasson; Andrew Graham; Jean-François Blouin; Leon Solomon; Jacques Gresset; Marc Melillo; Kenneth A Polse
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Eye & contact lens     Volume:  31     ISSN:  1542-2321     ISO Abbreviation:  Eye Contact Lens     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-15     Completed Date:  2005-10-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101160941     Medline TA:  Eye Contact Lens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158-65     Citation Subset:  IM    
School of Optometry and Research Unit in Vision Health, University of Montréal, Montréal, Canada.
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MeSH Terms
Case-Control Studies
Cell Shape
Cell Size
Corneal Diseases / pathology*
Diagnosis, Computer-Assisted / methods*
Endothelium, Corneal / pathology*
Reproducibility of Results
Grant Support

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