Document Detail


Morphometrical and microdensitometrical studies on phenylethanolamine-N-methyltransferase- and neuropeptide Y-immunoreactive neurons in the rostral medulla oblongata of the adult and old male rat.
MedLine Citation:
PMID:  3173685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present paper the neuronal systems of the medulla oblongata containing phenylethanolamine-N-methyltransferase- and neuropeptide Y-like immunoreactivity have been characterized in adult (3-month-old) and old (24-month-old) male rats. The phenylethanolamine-N-methyltransferase and neuropeptide Y-immunoreactive neurons have been visualized by means of immunocytochemistry (peroxidase-antiperoxidase technique) and analysed in a quantitative fashion by means of morphometrical (phenylethanolamine-N-methyltransferase- and neuropeptide Y-immunoreactive cell groups) and microdensitometrical (phenylethanolamine-N-methyltransferase-immunoreactive cell groups) approaches developed on the IBAS II image analyser (Zeiss-Kontron). During aging there is (a) a reduction in the area covered by the phenylethanolamine-N-methyltransferase-immunoreactive neuropil for both the C1 and C2 adrenaline cell groups; (b) a reduction in the area covered by the phenylethanolamine-N-methyltransferase-immunoreactive cell bodies, which is highly significant only for the C2 cell group; (c) a decrease in the area covered by the phenylethanolamine-N-methyltransferase-positive cell cluster for both C1 and C2 cell groups; (d) a decrease in the degree of phenylethanolamine-N-methyltransferase immunoreactivity present in the C1 and C2 cell groups; (e) a decay of neuropeptide Y immunoreactivity in the C1 and C2 groups, while the C3 group is unaffected by aging as evaluated by number of phenylethanolamine-N-methyltransferase- and neuropeptide Y-immunoreactive cell body profiles. These results indicate heterogeneities in the responses of the adrenaline-neuropeptide Y cell groups to the aging process. The possible functional consequences of aging-induced changes in the cardiovascular adrenergic neurons are discussed, especially in relation to development of hypertension.
Authors:
L F Agnati; K Fuxe; M Zoli; I Zini; A Härfstrand; G Toffano; M Goldstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience     Volume:  26     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-11-23     Completed Date:  1988-11-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  461-78     Citation Subset:  IM    
Affiliation:
Department of Human Physiology, University of Modena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism*
Animals
Epinephrine / metabolism*,  physiology
Image Processing, Computer-Assisted
Immunohistochemistry
Male
Medulla Oblongata / metabolism*,  physiology
Neuropeptide Y / metabolism*,  physiology
Phenylethanolamine N-Methyltransferase / metabolism*,  physiology
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
MH25504/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Neuropeptide Y; 51-43-4/Epinephrine; EC 2.1.1.28/Phenylethanolamine N-Methyltransferase

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