Document Detail

Morphometric and ultrastructural analysis of human granulosa cells after gonadotrophin-releasing hormone agonist or antagonist.
MedLine Citation:
PMID:  20335069     Owner:  NLM     Status:  MEDLINE    
Morphological features of granulosa cells can reflect their functional status. The present study was aimed at comparing possible differences in the fine structure of human granulosa cells exposed to gonadotrophin-releasing hormone (GnRH) agonist or antagonist treatment during ovarian stimulation. Cells were obtained from follicular aspirates of 21 women treated with recombinant follicle-stimulating hormone (rFSH) plus either a GnRH agonist or a GnRH antagonist. Conventional light microscopy procedures and computerized image analysis systems were used to identify different cell type morphological patterns and to quantify different cells distribution. Two morphologically distinct granulosa cell populations, defined as large/pale and small/dark cells, were identified and a different distribution in the two groups of women under investigation was found: a significantly higher percentage in large/pale cells was detected in the agonist-treated women (P<0.05), whereas the percentage of small/dark cells was significantly higher in the antagonist-treated group (P<0.05). Ultrastructural observations showed the presence in both cell populations of typical hallmarks of steroidogenic cells, highlighting signs of functional activity in the large/pale cell population. Further investigations are needed to define the possible clinical significance of these morphological findings.
L Centurione; F Giampietro; S Sancilio; M Piccirilli; L Artese; G M Tiboni; R Di Pietro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-12
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-30     Completed Date:  2011-01-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  625-33     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Department of Biomorphology, University G D'Annunzio Chieti-Pescara, Via dei Vestini 31, Chieti Scalo (CH), Italy.
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MeSH Terms
Follicle Stimulating Hormone / pharmacology
Gonadotropin-Releasing Hormone / agonists*,  antagonists & inhibitors*
Granulosa Cells / cytology,  drug effects*,  ultrastructure
Microscopy, Electron
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Recombinant Proteins; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-68-0/Follicle Stimulating Hormone

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