Document Detail

Morphometric and ultrastructural analysis of human granulosa cells after gonadotrophin-releasing hormone agonist or antagonist.
MedLine Citation:
PMID:  20335069     Owner:  NLM     Status:  MEDLINE    
Morphological features of granulosa cells can reflect their functional status. The present study was aimed at comparing possible differences in the fine structure of human granulosa cells exposed to gonadotrophin-releasing hormone (GnRH) agonist or antagonist treatment during ovarian stimulation. Cells were obtained from follicular aspirates of 21 women treated with recombinant follicle-stimulating hormone (rFSH) plus either a GnRH agonist or a GnRH antagonist. Conventional light microscopy procedures and computerized image analysis systems were used to identify different cell type morphological patterns and to quantify different cells distribution. Two morphologically distinct granulosa cell populations, defined as large/pale and small/dark cells, were identified and a different distribution in the two groups of women under investigation was found: a significantly higher percentage in large/pale cells was detected in the agonist-treated women (P<0.05), whereas the percentage of small/dark cells was significantly higher in the antagonist-treated group (P<0.05). Ultrastructural observations showed the presence in both cell populations of typical hallmarks of steroidogenic cells, highlighting signs of functional activity in the large/pale cell population. Further investigations are needed to define the possible clinical significance of these morphological findings.
L Centurione; F Giampietro; S Sancilio; M Piccirilli; L Artese; G M Tiboni; R Di Pietro
Related Documents :
18675449 - Preliminary assessment of somatic cell nuclear transfer in the dromedary (camelus drome...
9418999 - Proliferative activity of preovulatory follicles and newly formed corpora lutea in cycl...
2115479 - Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oo...
12096899 - Colocalization of heparin and histamine in the intracellular granules of test cells fro...
11165009 - Dynamics of the membrana granulosa during expansion of the ovarian follicular antrum.
11098029 - In-vitro ovarian steroidogenesis in women with pelvic congestion.
22467019 - Microarray and gene ontology analyses reveal downregulation of dna repair and apoptotic...
10204469 - Thrombin activates the growth, cell-cycle kinetics, and clustering of human dental pulp...
24233229 - Stable transformation of maize: the impact of feeder cells on protoplast growth and tra...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-12
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-30     Completed Date:  2011-01-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  625-33     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Department of Biomorphology, University G D'Annunzio Chieti-Pescara, Via dei Vestini 31, Chieti Scalo (CH), Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Follicle Stimulating Hormone / pharmacology
Gonadotropin-Releasing Hormone / agonists*,  antagonists & inhibitors*
Granulosa Cells / cytology,  drug effects*,  ultrastructure
Microscopy, Electron
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Recombinant Proteins; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mesenchymal stem cell therapy: Two steps forward, one step back.
Next Document:  Clopidogrel plus aspirin versus aspirin alone for reducing embolisation in patients with acute sympt...