| Morphometric study of colonic biopsies: a new method of estimating inflammatory diseases. | |
| | |
MedLine Citation:
|
PMID: 2733385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this study, colonic biopsy specimens from 87 subjects (37 men and 50 women; mean age 51 +/- 14 years) were investigated by a new method of quantitation morphometric analysis. Subjects were classified in 3 groups: I, control group (25 cases); II, constipation and/or abdominal pain (36 cases); III, diarrhea (26 cases). All biopsy specimens were considered normal by both colonoscopic and microscopic observations. Morphometric analysis was performed with a specialized automated image processor. Complete analysis of 1 biopsy specimen required about 10 minutes. Glandular area showed no difference in the ascending, transverse, and descending colonic sites in the three groups. By contrast, the rectal glandular area was significantly larger than those from the 3 colonic sites in all groups (p less than 0.001). The mean cellular density in the control group was 172.7 +/- 24 nuclei/unit area of lamina propria at all biopsy sites. The cellular densities of groups II and III were significantly increased in the ascending colon as compared with the group I subjects (195.1 +/- 22.8, p less than 0.01 and 198.2 +/- 24.7 p less than 0.001, respectively). The cellular density in group III was significantly increased in the other sites as compared with group I (p less than 0.01). Morphometric analysis does provide a definition of the normal range of the lamina propria cellularity and can identify mild abnormalities in patients in whose both colonoscopy and conventional histologic interpretation have been considered as normal by experienced endoscopists and pathologists. A statistical increase in cellular density in groups of patients with clinical symptoms should be regarded as consistent with the concept of microscopic colitis and detectable by morphometry. Such a method reduces interobserver bias and permits objective comparison of groups. |
| | |
Authors:
|
J L Salzmann; F Peltier-Koch; F Bloch; J P Petite; J P Camilleri |
Related Documents
:
|
20361745 - (2-nitrophenyl)acetyl: a new, selectively removable hydroxyl protecting group. 3415165 - Prognostic factors in extensive mesenteric ischaemia. 20411425 - Limited low-air insufflation is optimal for colonoscopy. 20401535 - The impact of advances in instrumentation and techniques of colonoscopy from 1988 to 20... 949025 - The natural history of small atrial septal defects; long-term follow-up with serial hea... 14656565 - Lethal post-tonsillectomy hemorrhage. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Laboratory investigation; a journal of technical methods and pathology Volume: 60 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1989 Jun |
Date Detail:
|
Created Date: 1989-07-21 Completed Date: 1989-07-21 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 847-51 Citation Subset: IM |
Affiliation:
|
Laboratoire d'Anatomie-Pathologique, Hopital Broussais, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biopsy Colic / pathology Colitis / pathology* Colon / pathology* Colonoscopy Constipation / pathology Diarrhea / pathology Female Humans Image Processing, Computer-Assisted Male Middle Aged |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Morphometric evaluation of the renal arterial system of Dahl salt-sensitive and salt-resistant rats ...
Next Document: Radioimmunoassay of buprenorphine in urine: studies in patients and in a drug clinic.