Document Detail


Morphometric characteristics of cell proliferation and p53 expression in development of experimentally induced respiratory tumors.
MedLine Citation:
PMID:  11531142     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study, under controlled conditions, the applicability of automated image analysis of immunohistochemical markers as an indicator of development and progression in tobacco component-induced tumors in the respiratory tract.
STUDY DESIGN: Amount, location, size, shape and intensity of staining of proliferating cell and p53 antigen in chemically induced precursors and squamous cell carcinoma of the hamster lung were determined by computer-assisted morphometry.
RESULTS: The total expression of proliferating cell nuclear antigen (PCNA) and p53 expression increased consistently during the formation of papillomas and squamous cell carcinomas of the larynx, trachea, bronchi and lungs. Individual preneoplastic cells in epithelial dysplasia expressed PCNA staining, increasing with increasing cell size and optical density, indicating antibody- staining intensity, in relation to the increased degree of cellular atypia. In malignant tumors, cell size decreased with decreasing differentiation, while antibody staining intensity remained unchanged. The increased alterations in cell shape and percent PCNA-positive cells observed in dysplastic epithelium and squamous cell carcinomas were statistically significant using Spearman's correlation coefficient. Squamous cell carcinomas consisted of two tumor cell populations with different cell shapes, and PCNA and p53 staining intensity. Altering measurement conditions-antibody threshold levels, size of measured area and repeating measurements-showed computer-assisted image analysis to give sensitive, reliable and consistent results.
CONCLUSION: Computer-assisted analysis of immunohistochemical staining showed high sensitivity and reproducibility; however, the results depended upon the method of study.
Authors:
J Laitakari; D Harrison; F Stenbäck
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  23     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-09-03     Completed Date:  2002-01-28     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbazoles / chemistry,  pharmacology
Carcinoma, Squamous Cell / etiology,  metabolism,  pathology*
Cell Division*
Cell Size
Cricetinae
Gene Expression
Image Processing, Computer-Assisted* / methods
Immunohistochemistry
Mesocricetus
Papilloma / etiology,  metabolism,  pathology*
Pilot Projects
Precancerous Conditions / metabolism,  pathology
Proliferating Cell Nuclear Antigen / analysis
Reproducibility of Results
Respiratory Tract Neoplasms / etiology,  metabolism*,  pathology*
Sensitivity and Specificity
Staining and Labeling
Tumor Suppressor Protein p53 / metabolism*
Chemical
Reg. No./Substance:
0/Carbazoles; 0/Proliferating Cell Nuclear Antigen; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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